Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

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文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

中文名稱(chēng)：

小鼠髓過(guò)氧化物酶特異性抗中性粒細(xì)胞胞質(zhì)抗體酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08676m
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠髓過(guò)氧化物酶特異性抗中性粒細(xì)胞胞質(zhì)抗體酶聯(lián)免疫試劑盒（CSB-E08676m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的MAC Ab含量。小鼠髓過(guò)氧化物酶（MPO）特異性抗中性粒細(xì)胞胞質(zhì)抗體（ANCA）與血管炎密切相關(guān)，感染可能引發(fā)MPO-ANCA產(chǎn)生，進(jìn)而誘發(fā)血管炎。MPO-ANCA通過(guò)誘導(dǎo)中性粒細(xì)胞活化及形成中性粒細(xì)胞外捕獲物（NETs）促進(jìn)ANCA產(chǎn)生，NETs中包含MPO，可能觸發(fā)機(jī)體產(chǎn)生自身抗體導(dǎo)致血管炎。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于科研領(lǐng)域探究小鼠模型中MPO - ANCA的動(dòng)態(tài)變化，如評(píng)估炎癥性疾病進(jìn)展、自身免疫模型構(gòu)建或藥物干預(yù)效果研究。支持科研人員在體外實(shí)驗(yàn)中高效獲取穩(wěn)定可靠的實(shí)驗(yàn)數(shù)據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

MAC Ab
種屬：

Mus musculus (Mouse)
樣本類(lèi)型：

serum, plasma
檢測(cè)范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	95
	Range %	83-101
1:4	Average %	96
	Range %	90-106
1:8	Average %	94
	Range %	82-101

回收率：

The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA)spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.


Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-98
EDTA plasma (n=4)	94	91-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.404	2.418	2.411	2.297
20	1.956	1.904	1.930	1.816
10	1.352	1.377	1.365	1.251
5	0.902	0.934	0.918	0.804
2.5	0.526	0.534	0.530	0.416
1.25	0.311	0.326	0.319	0.205
0.625	0.265	0.271	0.268	0.154
0	0.112	0.115	0.114

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity Sangaletti S et al,Blood,2012

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.