Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

產品詳情

文獻引用 (1) 評價 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠白介素18結合蛋白(IL-18BP)酶聯(lián)免疫試劑盒（CSB-E17797m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的IL-18BP含量。IL-18BP（白介素-18結合蛋白）是一種免疫檢查點蛋白，可抑制IL-18與其受體結合，調節(jié)Th1細胞因子反應。其在多種腫瘤和自身免疫性疾病中高表達，研究機制涉及IL-18與IL-18BP的相互作用及其對免疫反應的影響。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，科研人員可將其應用于炎癥性疾病動物模型的機制研究、藥物干預效果評估或體外細胞實驗中IL-18/IL-18BP信號通路的動態(tài)監(jiān)測，特別適用于探索類風濕性關節(jié)炎、炎癥性腸病等病理過程中IL-18通路的調控機制本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit
縮寫：

IL-18BP
Uniprot No.：

Q9Z0M9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	84
	Range %	80-88
1:4	Average %	104
	Range %	100-107
1:8	Average %	91
	Range %	87-95

回收率：

The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	89	84-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 2 個評價

樣品類型：組織勻漿液小鼠結腸

樣品信息：小鼠

稀釋比：其他稀釋2倍

產品評價：我用了貨號為CSB-E17797m的Mouse Interleukin-18 Binding Prorein (IL-18BP)ELISA Kit檢測了DSS誘導結腸炎之后小鼠結腸組織勻漿中的IL-18BP蛋白，OD值為2左右，該試劑盒效果不錯，反應靈敏，達到了預期實驗的目的

By 胡老師

樣品類型：血清

樣品信息：小鼠

稀釋比：沒有稀釋

產品評價：我用CSB-E17797m檢測正常小鼠和造模狼瘡小鼠的血清，未稀釋樣品進行檢測。標準品OD450值最高為1.99，最低為0.02，標準曲線R平方大于0.99。小鼠血清OD450值范圍約為0.3-1.3左右, 對應濃度為1.5-7ng/mL, 符合我的預期濃度范圍。

By 唐老師

功能：

Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
基因功能參考文獻：
1. IL-18BP ameliorates cardiac ischemia/reperfusion injury in part through suppression of Th17 differentiation. PMID: 26340315
2. Data show that IL-18bp acts as a sensor for IFN-gamma and can regulate both Th1 and Th17 responses in the CNS. PMID: 20644165
3. Administratioan of murine IL-18BP at the time of the booster injection of bovine CII reduces the progressive severity of collagen-induced arthritis; both clinical disease activity and histological scores are decreased by 50%. PMID: 12574381
4. functional polarization of Ag-specific CD4(+) Th2 cells entering the CNS during the accelerating phase of experimental autoimmune encephalomyelitis is flexible and dependent on IL-18, which is overturned by IL-18 binding protein PMID: 15778395
5. IL-18 and IL-18BP may have roles in testicular development, and also in the regulation of testicular functions under physiological conditions PMID: 18299271
6. A single intravenous dose of recombinant mouse IL18BP decreased neointimal hyperplasia and improved arterial L/A ratios in an atherosclerotic balloon-injury animal model. PMID: 18455646
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亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

KEGG: mmu:16068

STRING: 10090.ENSMUSP00000091685

UniGene: Mm.422756