Mouse fibrin ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血纖蛋白(fibrin)酶聯(lián)免疫試劑盒（CSB-EQ027562MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的fibrin含量。Fibrin即纖維蛋白，是凝血過程的關(guān)鍵產(chǎn)物。其背景與止血和血栓形成密切相關(guān)。研究機(jī)制上，聚焦于其在凝血級聯(lián)反應(yīng)中的生成過程，以及與血小板相互作用形成血栓的機(jī)制，還探索其在疾病如心血管病、炎癥等病理過程中的作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，可用于研究凝血機(jī)制異常、心血管疾病模型中的纖維蛋白沉積動態(tài)，或評估抗血栓藥物對纖維蛋白生成的干預(yù)效果，亦可用于炎癥性疾病或組織損傷模型中纖維蛋白代謝通路的分子機(jī)制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

fibrin
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse fibrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	87-103
1:200	Average %	96
	Range %	86-102
1:400	Average %	92
	Range %	84-100
1:800	Average %	94
	Range %	85-100

回收率：

The recovery of mouse fibrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-101
EDTA plasma (n=4)	95	89-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.