Mouse apolipoprotein H (Apo-H) ELISA Kit

Mouse apolipoprotein H (Apo-H) ELISA Kit

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中文名稱：

小鼠載脂蛋白H(Apo-H)酶聯免疫試劑盒
貨號：

CSB-E10074m
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠載脂蛋白H(Apo-H)酶聯免疫試劑盒（CSB-E10074m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOH含量。APOH即載脂蛋白H，又稱β2-糖蛋白 I。它參與細胞內吞、凝血調節等生理過程。研究發現，APOH和自身免疫病、心血管疾病等密切相關，其通過與多種配體相互作用，影響信號傳導和免疫反應，是疾病機制和藥物研發的潛在靶點。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于小鼠模型中的脂代謝研究、自身免疫性疾病機制探索或心血管相關實驗的樣本分析；助力于脂蛋白功能研究、疾病動物模型構建及藥物干預效果評價等基礎科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Apoh ELISA kit; B2gp1Beta-2-glycoprotein 1 ELISA kit; APC inhibitor ELISA kit; Activated protein C-binding protein ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
縮寫：

APOH
Uniprot No.：

Q01339
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	83-92
1:2000	Average %	97
	Range %	91-101
1:4000	Average %	89
	Range %	84-92
1:8000	Average %	97
	Range %	91-101

回收率：

The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	103	98-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.669	2.625	2.647	2.512
500	2.180	2.112	2.146	2.011
250	1.611	1.600	1.606	1.471
125	0.859	0.831	0.845	0.710
62.5	0.515	0.527	0.521	0.386
31.2	0.321	0.333	0.327	0.192
15.6	0.248	0.242	0.245	0.110
0	0.136	0.134	0.135

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
基因功能參考文獻：
1. During the parasite's hepatic replication, the C-terminal region of EXP-1 binds ApoH. This molecular interaction plays a pivotal role for successful Plasmodium liver-stage development. Down-regulation of ApoH expression in either hepatic cells or mouse livers by RNA interference resulted in impaired intrahepatic development. PMID: 28137845
2. A1-A1, which is a soluble analog of ApoER2 that binds pathological beta2GPI/anti-beta2GPI complexes, has a positive impact on hemodynamics in lupus-prone mice with spontaneous anti-beta2GPI antibodies and hypertension PMID: 26990752
3. These results suggested that CRP/oxidized low density lipoprotein/beta 2-Glycoprotein I complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFkappaB signaling pathways. PMID: 27512978
4. TLR2 increase the stimulating effect of beta2GP1/anti-beta2GP1 complex on the expression of TNF-alpha in mouse peritoneal macrophages PMID: 27053607
5. The Fifth Domain of Beta 2 Glycoprotein I Protects from Natural IgM Mediated Cardiac Ischaemia Reperfusion Injury. PMID: 27031114
6. These results indicate that both NF-kappaB and c-Jun/AP-1 are involved in regulating anti-beta2GPI-induced expression of prothrombotic and proinflammatory molecules in vivo. PMID: 26829121
7. Phospholipid scramblase 1 regulates phosphatidylserine exposure in response to oxygen stress, leading to beta2-glycoprotein I and IgM binding and lipid-mediated, inflammatory responses. PMID: 26216936
8. beta2-GPI-D-I-tDCs may offer a novel approach for developing therapy for APS patients PMID: 24972993
9. Data suggest that factors enabling a beta2-glycoprotein I (beta2GPI)-reactive T cell response may predispose individuals to the development of systemic lupus erythematosus (SLE)-related autoantibodies PMID: 25555913
10. Deletion of beta2 GPI accelerates and potentiates the autoimmune phenotype in male BXSB.Yaa mice PMID: 24692206
11. beta2-glycoprotein I-derived peptides attenuate injury and inflammation when administered in a therapeutic manner in intestinal ischemia/reperfusion injury. PMID: 23034168
12. Interaction of beta2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages PMID: 21965665
13. beta2GPI binding to EC requires priming with pro-inflammatory factors which is not needed for uterine and placental localization probably dependent on hormonal changes. PMID: 21791419
14. gene knock-out mice display enhanced B16 melanoma development and tumor microvessel density PMID: 20655705
15. Endothelial cell incubation with anti-lysobisphosphatidic-acid MoAb or with patients' IgG resulted in antibody binding to late endosomes and caused beta2GPI redistribution and accumulation in perinuclear vesicular structures reminiscent of late endosomes. PMID: 12008959
16. Data are consistent with pathogenic mechanisms in antiphospholipid syndrome involving both neutralization of beta(2)-glycoprotein I (beta(2)GPI) function and beta(2)GPI-immunoglobulin complex formation. PMID: 15100383
17. beta-2-glycoprotein 1 plays an important physiologic role in the recognition of dying cells PMID: 15843904
18. certain subpopulations of antibodies raised against tetanus toxoid and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential PMID: 19086991
19. These results are compatible with a role for apolipoproteins in lipid metabolism and transport in the developing lung in association with the sex difference in surfactant lipid synthesis. PMID: 19106236
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亞細胞定位：

Secreted.
組織特異性：

Expressed by the liver and secreted in plasma.
數據庫鏈接：

KEGG: mmu:11818

STRING: 10090.ENSMUSP00000000049

UniGene: Mm.2266

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.