Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

文獻引用 (1) 說明書下載

中文名稱：

小鼠α1微球蛋白/bikunin蛋白前體(AMBP)酶聯免疫試劑盒
貨號：

CSB-E09776m
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
靶點詳情

產品詳情

產品描述：

小鼠α1微球蛋白/bikunin蛋白前體(AMBP)酶聯免疫試劑盒（CSB-E09776m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AMBP含量。AMBP是一個靶點。它是血管緊張素原結合蛋白，在血壓調節等生理過程中發揮作用。研究機制方面，可能涉及與血管緊張素等的相互作用來影響心血管系統。但目前相關研究或許還在不斷深入探索其在疾病發生發展中的具體角色和潛在應用。試劑盒檢測范圍為1.563 ng/mL-100 ng/mL，本試劑盒適用于基礎科研中探索AMBP在急慢性炎癥、組織損傷修復或代謝性疾病模型中的動態變化，亦可用于評估藥物干預或基因修飾對AMBP表達的影響。助力于腎臟生理學、肝臟病理學及炎癥調控機制等領域的研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
縮寫：

AMBP
Uniprot No.：

Q07456
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.563 ng/mL-100 ng/mL
靈敏度：

0.649 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	104
	Range %	101-107
1:200	Average %	96
	Range %	93-99
1:400	Average %	94
	Range %	90-97
1:800	Average %	91
	Range %	84-95

回收率：

The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	97	92-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
100	0.140	0.145	0.143
50	0.357	0.366	0.362
25	0.569	0.578	0.574
12.5	0.865	0.898	0.882
6.25	1.544	1.532	1.538
3.125	2.083	2.098	2.091
1.563	2.469	2.466	2.468
0	2.844	2.878	2.861

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Sirtuin 6 affects glucose reabsorption and gluconeogenesis in type 1 diabetes via FoxO1 Che Bian,Molecular and Cellular Endocrinology,2022

產品評價

靶點詳情

功能：

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.; Trypstatin is a trypsin inhibitor.
基因功能參考文獻：
1. Loss of bikunin expression is associated with Embryos failing to Initiate Midgut Rotation. PMID: 30174180
2. In this review, the use of genetically targeted mice selectively lacking UTI provides evidence of a therapeutic option for experimental endotoxin-related inflammatory disorders such as acute lung injury and acute liver injury. PMID: 20367192
3. expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in embryogenesis PMID: 12204273
4. UTI inhibited LPS-induced production of TNF-alpha by inhibiting activation of the extracellular signal-regulated protein kinases 1/2-Egr-1 pathway in monocytes, which might at least partly contribute to reduction of hypotension PMID: 15539418
5. UTI protects against systemic inflammatory response and subsequent organ injury induced by bacterial endotoxin. PMID: 15576631
6. results indicate that endogenous UTI is protective against acute lung injury induced by bacterial endotoxin, at least partly, via the antioxidative properties PMID: 16273282
7. increased sensitivity of Bik-/- mice to lung metastasis in vivo is due to a lack of circulating proteins of the inter-alpha inhibitor family, especially bikunin PMID: 16331631
8. UTI reduces NF-kappaB activation and downregulates the expression of its related mediators, followed by the inhibition of neutrophil aggregation and infiltration in hepatic ischemia reperfusion injury PMID: 19208516
9. Report impaired liver regeneration with humoral and genetic disturbances in urinary trypsin inhibitor-deficient mice. PMID: 19302180
10. UTI protects against severe liver injury and subsequent coagulatory disturbance which was mediated, at least partly, through the suppression of TNF-alpha production along with its antiprotease activity. PMID: 19398962
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亞細胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
組織特異性：

Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
數據庫鏈接：

KEGG: mmu:11699

STRING: 10090.ENSMUSP00000030041

UniGene: Mm.2197

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