Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

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文獻引用 (1) 說明書下載

中文名稱：

小鼠WAP四-二硫核心結構域蛋白2(WFDC2)酶聯免疫試劑盒
貨號：

CSB-EL026092MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠WAP四-二硫核心結構域蛋白2(WFDC2)酶聯免疫試劑盒（CSB-EL026092MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的WFDC2含量。小鼠WAP四-二硫核心結構域蛋白2(WFDC2)是一種分泌蛋白，含有兩個WAP結構域，主要在男性生殖系統、呼吸道和鼻咽部表達。其在多種腫瘤細胞系中高表達，可能參與腫瘤的發生發展，并與支氣管擴張有關，其研究機制涉及基因變異和蛋白質功能。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于基礎科研中探索WFDC2在疾病模型（如炎癥性疾病、纖維化病變或代謝紊亂）中的生物學功能，分析其在組織損傷修復中的動態變化，或評估特定干預措施對WFDC2表達水平的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Wfdc2 ELISA Kit; He4 ELISA Kit; WAP four-disulfide core domain protein 2 ELISA Kit; WAP domain-containing protein HE4 ELISA Kit
縮寫：

WFDC2
Uniprot No.：

Q9DAU7
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse WFDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-95
1:2	Average %	96
	Range %	90-102
1:4	Average %	97
	Range %	92-105
1:8	Average %	95
	Range %	89-98

回收率：

The recovery of mouse WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	92	86-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.045	2.144	2.095	1.943
2000	1.624	1.743	1.684	1.532
1000	1.053	1.072	1.063	0.911
500	0.673	0.663	0.668	0.516
250	0.342	0.351	0.347	0.195
125	0.274	0.283	0.279	0.127
62.5	0.229	0.235	0.232	0.080
0	0.152	0.151	0.152

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Synergistic effect of Tripterygium glycosides and cisplatin on drug-resistant human epithelial ovarian cancer via ILK/GSK3β/Slug signal pathway Y Yu,American journal of translational research,2022

■ 常見問題解答

I have a few questions on product CSB-EL026092MO ：
1. Which is this standard in this kit, natural material or recombinant?
If recombinant, please let us know expression method(E.coli etc.).
2. Is the standard or antibody, including any additive agent(antiseptic
agent or preserving agent)? If so, please let us know composition and
concentration.
3. Is this product including Stop solution? If so, please tell us
composition and concentration for stop solution.
4. Please let us know whether this kit include any hazardous
substances(azide, thimerosal, 2-ME, etc). If so, please let us know
composition and concentration.

Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EL026092MO is NS0-derived recombinant protein
2,Preservative of this kit is proclin 300(1:3000)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.

收起更多

功能：

Broad range protease inhibitor.
基因功能參考文獻：
1. Causal testing implicates Wfdc2 and Hdac3 as novel genes affecting high density lipoprotein levels PMID: 21807117
亞細胞定位：

Secreted.
數據庫鏈接：

KEGG: mmu:67701

STRING: 10090.ENSMUSP00000017867

UniGene: Mm.27289

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.