Mouse Tetranectin(CLEC3B) ELISA kit

說明書下載

產品詳情

產品描述：

小鼠四連接素(CLEC3B)酶聯(lián)免疫試劑盒（CSB-EL005531MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CLEC3B含量。CLEC3B，也稱為四連蛋白，是一種分泌的同源三聚體蛋白，主要存在于血漿中。它能夠與纖溶酶原和kringle 4結合，可能參與胞吐作用的分子包裝。CLEC3B的表達與癌癥進展和不良預后相關，可能通過調節(jié)免疫細胞浸潤和抑制細胞增殖來影響腫瘤發(fā)展。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產品廣泛應用于腫瘤生物學、細胞信號轉導等領域的實驗研究，特別適用于基因敲除/過表達模型的蛋白表達驗證、疾病動物模型的分子標志物動態(tài)監(jiān)測等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

Clec3b ELISA Kit; Tna ELISA Kit; Tetranectin ELISA Kit; TN ELISA Kit; C-type lectin domain family 3 member B ELISA Kit; Plasminogen kringle 4-binding protein ELISA Kit
縮寫：

CLEC3B
Uniprot No.：

P43025
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	104
	Range %	100-112
1:200	Average %	90
	Range %	86-94
1:400	Average %	86
	Range %	82-90
1:800	Average %	93
	Range %	89-98

回收率：

The recovery of mouse CLEC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
亞細胞定位：

Secreted.
組織特異性：

Highest expression in lung and skeletal muscle.
數(shù)據庫鏈接：

STRING: 10090.ENSMUSP00000026890

UniGene: Mm.34588