Mouse Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

Mouse Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

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中文名稱：

小鼠破骨細胞相關免疫球蛋白樣受體(OSCAR)酶聯免疫試劑盒
貨號：

CSB-EL017255MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠破骨細胞相關免疫球蛋白樣受體(OSCAR)酶聯免疫試劑盒（CSB-EL017255MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OSCAR含量。小鼠破骨細胞相關免疫球蛋白樣受體(OSCAR)在破骨細胞形成和免疫調節中起關鍵作用，能促進破骨細胞形成及存活，并參與免疫細胞的激活和成熟，是骨重建和免疫治療的重要靶點。試劑盒檢測范圍為37.5 pg/mL-2400 pg/mL，支持科研人員開展骨代謝調控機制、骨質疏松模型評估、骨免疫相關藥物開發等基礎研究，為體外實驗提供可靠的定量數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Oscar ELISA Kit; Osteoclast-associated immunoglobulin-like receptor ELISA Kit; Osteoclast-associated receptor ELISA Kit; mOSCAR ELISA Kit; Ocl178 ELISA Kit
縮寫：

OSCAR
Uniprot No.：

Q8VBT3
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

37.5 pg/mL-2400 pg/mL
靈敏度：

9.375 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSCAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
Range %	82-93
1:10	Average %	105
Range %	97-108
1:20	Average %	93
Range %	88-97
1:40	Average %	95
Range %	91-98

回收率：

The recovery of mouse OSCAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	91	85-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.885	2.877	2.881	2.761
1200	2.068	2.089	2.079	1.959
600	1.152	1.123	1.138	1.018
300	0.689	0.687	0.688	0.568
150	0.398	0.391	0.395	0.275
75	0.301	0.289	0.295	0.175
37.5	0.210	0.222	0.216	0.096
0	0.119	0.121	0.120

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

A novel autoantibody signatures for enhanced clinical diagnosis of pancreatic ductal adenocarcinoma T Li,Cancer cell international,2023
Caffeic Acid Phenethyl Ester Abrogates Bone Resorption in a Murine Calvarial Model of Polyethylene Particle-Induced Osteolysis Zawawi MS et al,Calcif Tissue Int,2015

功能：

Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
基因功能參考文獻：
1. OSCAR expression in monocytic cells is regulated by proatherogenic stimuli further pointing towards a role in vascular inflammation or plaque vulnerability during atherosclerosis PMID: 23817038
2. Data suggest that OSCAR is a collagen receptor that binds to specific collagen motifs and costimulates osteoclastogenesis in DAP12-deficient humans and mice. PMID: 21841309
3. PU.1 and MITF transcription factors synergistically activate OSCAR gene expression, which is further enhanced by the TRANCE-induced MKK6/p38 signaling cascade. PMID: 12695521
4. the FcRgamma-mediated signal transduction by OSCAR is involved in osteoclast differentiation PMID: 15184345
5. analysis of a positive feedback circuit of TRANCE-induced activation of NFATc1, involving NFATc1-mediated OSCAR expression and its subsequent activation of NFATc1, necessary for efficient differentiation of osteoclasts PMID: 16109714
6. Results indicate that USFs 1 and 2 serve as modulators in the induction of OSCAR and RANKL-mediated osteoclastogenesis. PMID: 18762192
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Leukocyte receptor complex/polymeric immunogobulin receptor (PIR/LRC) family
組織特異性：

Specifically expressed in preosteoclasts or mature osteoclasts.
數據庫鏈接：

KEGG: mmu:232790

STRING: 10090.ENSMUSP00000041306

UniGene: Mm.358882

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.