Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

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中文名稱：

小鼠鳥氨酸氨甲酰基轉移酶，線粒體(OTC)酶聯免疫試劑盒
貨號：

CSB-EL017270MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠鳥氨酸氨甲酰基轉移酶，線粒體(OTC)酶聯免疫試劑盒（CSB-EL017270MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OTC含量。試劑盒檢測范圍為3.12 mU/mL-200 mU/mL，適用于肝病模型研究、尿素循環代謝機制探索、基因工程動物模型驗證等科研場景，可滿足科研實驗中定量分析OTC酶動態變化的需求，為代謝性疾病機制研究、藥物肝毒性評估等提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Otc ELISA Kit; Ornithine carbamoyltransferase ELISA Kit; mitochondrial ELISA Kit; EC 2.1.3.3 ELISA Kit; Ornithine transcarbamylase ELISA Kit; OTCase ELISA Kit
縮寫：

OTC
Uniprot No.：

P11725
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 mU/mL-200 mU/mL
靈敏度：

0.78 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of mouse OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-109
EDTA plasma (n=4)	96	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
200	2.348	2.272	2.310	2.196
100	1.826	1.732	1.779	1.665
50	1.176	1.239	1.208	1.094
25	0.708	0.743	0.726	0.612
12.5	0.409	0.431	0.420	0.306
6.25	0.257	0.271	0.264	0.150
3.12	0.199	0.204	0.202	0.088
0	0.115	0.112	0.114

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.
基因功能參考文獻：
1. Gene correction in adult OTC-deficient mice was lower and accompanied by larger deletions that ablated residual expression from the endogenous OTC gene, leading to diminished protein tolerance and lethal hyperammonemia on a chow diet PMID: 26829317
2. The corresponding OTC tissue enzyme activities were between 3-6% of normal control in mouse and human liver. The use of the cryptic splice sites was reproduced in minigenes carrying murine or human mutant sequences PMID: 25853564
3. Serum OCT seemed to reflect tumor necrosis factor-alpha-mediated hepatic damage in diabetic obese mice and could be useful in the application for non-alcoholic fatty liver disease with features of metabolic syndrome, such as obesity and diabetes. PMID: 19793175
4. in vivo regulation by HNF4alpha PMID: 11994307
5. Results highlight the importance of the interaction between the OTC spf-ash mutation (ornithine transcarbamylase deficiency) and genetic backgrounds on metabolic phenotypes (ureagenesis, arginine metabolism, and nitric oxide production). PMID: 17925451
6. Phenotypic correction of OTC deficiency using low-dose helper-dependent adenoviral vectors is reported. PMID: 18563850
7. Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals. PMID: 19318352
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相關疾病：

Defects in Otc are the cause of the Sparse fur (spf) phenotype. Spf mouse have an OTCase with an overall decrease in activity, and altered substrate affinity.
亞細胞定位：

Mitochondrion matrix.
蛋白家族：

ATCase/OTCase family
數據庫鏈接：

KEGG: mmu:18416

STRING: 10090.ENSMUSP00000056152

UniGene: Mm.2611

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.