Mouse Mucin-2,MUC2 ELISA Kit

Mouse Mucin-2,MUC2 ELISA Kit

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中文名稱：

小鼠粘蛋白/粘液素2(MUC2)酶聯(lián)免疫試劑盒
貨號：

CSB-E15065m
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠粘蛋白/粘液素2(MUC2)酶聯(lián)免疫試劑盒（CSB-E15065m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MUC2含量。MUC2是一種粘蛋白，在多種上皮組織表面發(fā)揮保護屏障作用，維持腸道穩(wěn)態(tài)。研究發(fā)現(xiàn)其表達異常與腫瘤、炎癥性腸病等疾病相關(guān)。機制上，它通過調(diào)節(jié)細胞黏附、信號傳導等過程影響疾病發(fā)生發(fā)展，可作為潛在治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，試劑盒適用于腸道炎癥模型研究、黏液分泌機制探索、黏膜屏障功能評估等科研領(lǐng)域，支持體外實驗和動物實驗中的動態(tài)監(jiān)測需求，為消化道疾病機制研究及藥物開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Muc2 ELISA Kit; Mucin-2 ELISA Kit; MUC-2 ELISA Kit; Colonic mucin ELISA Kit; MCM ELISA Kit; Secreted gel-forming mucin ELISA Kit; Fragments ELISA Kit
縮寫：

MUC2
Uniprot No.：

Q80Z19
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	90
	Range %	83-97
1:40	Average %	95
	Range %	86-107
1:80	Average %	95
	Range %	86-102
1:160	Average %	95
	Range %	88-103

回收率：

The recovery of mouse MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-103
EDTA plasma (n=4)	93	89-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.113	2.024	2.069	1.916
1000	1.626	1.685	1.656	1.503
500	1.105	1.166	1.136	0.983
250	0.643	0.673	0.658	0.505
125	0.458	0.481	0.470	0.317
62.5	0.319	0.337	0.328	0.175
31.25	0.252	0.262	0.257	0.104
0	0.152	0.154	0.153

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Lactobacillus strains reduce the toxic effects of a subchronic exposure to arsenite through drinking water A Domene,Environmental research,2023
Ficolin A knockout alleviates sepsis-induced severe lung injury in mice by restoring gut Akkermansia to inhibit S100A4/STAT3 pathway X Wu,International immunopharmacology,2023
Protective Effects and Mechanism of a Novel Probiotic Strain Ligilactobacillus salivarius YL20 against Cronobacter sakazakii-Induced Necrotizing Enterocolitis In Vitro and In Vivo W Wang,Nutrients,2022
Bacillus subtilis RZ001 improves intestinal integrity and alleviates colitis by inhibiting Notch signaling pathway and activating ATOH-1 Li Y,Pathogens and Disease,2020
Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice Song-ZiXie, et al,Carbohydrate Polymers,2018

功能：

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
亞細胞定位：

Secreted. Note=In the intestine, secreted into the inner and outer mucus layers.
組織特異性：

Highly expressed in goblet cells of the colon with lower levels in the small intestine and no expression in the stomach (at protein level).
數(shù)據(jù)庫鏈接：

STRING: 10090.ENSMUSP00000026590

UniGene: Mm.2041

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.