Mouse Leucine-rich repeat LGI family member 3(LGI3) ELISA kit

Mouse Leucine-rich repeat LGI family member 3(LGI3) ELISA kit

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中文名稱：

小鼠富亮氨酸重復LGI家族成員3(LGI3)酶聯免疫試劑盒
貨號：

CSB-EL012900MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠富亮氨酸重復LGI家族成員3(LGI3)酶聯免疫試劑盒（CSB-EL012900MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LGI3含量。小鼠富亮氨酸重復LGI家族成員3（LGI3）是一種神經生物學相關蛋白，參與調節神經元外排作用，主要在腦和肺中表達。其功能可能與抑制性突觸組裝有關，對神經科學領域的研究具有重要意義。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于神經科學基礎研究，可應用于腦組織、外周神經或疾病模型動物血清/血漿中LGI3蛋白表達的定量分析，為探究神經發育異常、癲癇發病機制及藥物干預效果提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Lgi3Leucine-rich repeat LGI family member 3 ELISA Kit; Leubrin ELISA Kit; Leucine-rich glioma-inactivated protein 3 ELISA Kit
縮寫：

LGI3
Uniprot No.：

Q8K406
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGI3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	87
	Range %	83-90
1:4	Average %	102
	Range %	98-108
1:8	Average %	89
	Range %	85-94

回收率：

The recovery of mouse LGI3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	108	104-112
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.106	2.138	2.122	1.950
1000	1.465	1.436	1.451	1.279
500	0.895	0.918	0.907	0.735
250	0.551	0.536	0.544	0.372
125	0.329	0.331	0.330	0.158
62.5	0.258	0.249	0.254	0.082
31.25	0.201	0.210	0.206	0.034
0	0.170	0.173	0.172

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The Suppressive Effect of Leucine-Rich Glioma Inactivated 3 (LGI3) Peptide on Impaired Skin Barrier Function in a Murine Model Atopic Dermatitis US Kim,Pharmaceutics,2020
Leucine-rich glioma inactivated 3 associates negatively with adiponectin Kim HA et al,Cytokine.,2013

功能：

May participate in the regulation of neuronal exocytosis.
基因功能參考文獻：
1. Taken together, these results support the critical involvement of LGI3 in the cytokine network by interplaying with multiple adipokines, cytokines and signaling proteins. PMID: 28534931
2. findings show LGI3 and TNF-alpha upregulated each other in 3T3-L1 cells; study suggests LGI3 is a pro-inflammatory adipokine and forms a positive feedback loop with TNF-alpha; propose that LGI3 is a member of adipokine network that plays regulatory roles in obesity-associated adipose tissue inflammation PMID: 25648289
3. results suggested that LGI3 may participate in adipose tissue homeostasis by negatively regulating adiponectin PMID: 23548727
4. Data suggest that leucine-rich glioma inactivated 3 (LGI3) may be a candidate adipokine that is perturbed in obesity and suppresses adipogenesis through its receptor, ADAM23. PMID: 22405860
5. LGI3 interacts with Flo1 in the brain, and LGI3/Flo1 mediates APP trafficking and exosome formation in neuronal cells: reveals the details of the intracellular transport system and even the secretion pathway in the brain. PMID: 20461023
6. we propose that LGI3 is a neuritogenic factor whose signaling pathway involves Akt-mediated FAK activation. PMID: 20162351
7. LGI3 gene widespread expression in the brain suggests that its transcripts might be involved in a common cellular process present in different neuronal types. PMID: 19833108
8. The expression of mouse LGI3 (mLGI3) in adult and developing brain and analyzed the 5'-upstream transcriptional regulatory regions of mLGI3 gene. PMID: 16545924
9. Data show that LGI3 localizes to the endocytic pathway and its accumulation is caused by endocytic perturbation. PMID: 18628660
10. LGI3 may play a regulatory role in neuronal exocytosis via its interaction with syntaxin 1. PMID: 18760330
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亞細胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cell junction, synapse, synaptosome. Note=Found in the synaptosomal membrane fraction.
組織特異性：

Brain.
數據庫鏈接：

KEGG: mmu:213469

STRING: 10090.ENSMUSP00000046705

UniGene: Mm.41708

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.