Mouse Keratinocyte Growth Factor(KGF/FGF-7) ELISA Kit
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中文名稱：小鼠角化細(xì)胞生長因子(KGF/FGF-7)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E13046m
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠角化細(xì)胞生長因子(KGF/FGF-7)酶聯(lián)免疫試劑盒（CSB-E13046m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的FGF7含量。FGF7，即成纖維細(xì)胞生長因子7，是一種上皮細(xì)胞特異性生長因子，主要參與組織發(fā)育、修復(fù)和維持。其研究機(jī)制涉及與FGFR2b受體結(jié)合，調(diào)節(jié)細(xì)胞增殖、分化和存活，并在多種生理過程中發(fā)揮關(guān)鍵作用。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，該產(chǎn)品可廣泛應(yīng)用于上皮細(xì)胞生物學(xué)、組織再生機(jī)制、傷口愈合模型等基礎(chǔ)研究領(lǐng)域，也可用于評(píng)估藥物干預(yù)或基因修飾對(duì)KGF表達(dá)水平的影響。對(duì)于細(xì)胞實(shí)驗(yàn)，可直接檢測培養(yǎng)上清中分泌型KGF的濃度；針對(duì)動(dòng)物模型，支持從血清、血漿或特定組織勻漿中定量分析內(nèi)源性KGF的表達(dá)動(dòng)態(tài)，為皮膚、肺、腸道等上皮組織相關(guān)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Fgf7 ELISA Kit; Fgf-7 ELISA Kit; Kgf ELISA Kit; Fibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit; KGF ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：1.56 pg/mL-100 pg/mL
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靈敏度：0.39 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 94 Range % 89-98 1:200 Average % 96 Range % 91-102 1:400 Average % 90 Range % 85-96 1:800 Average % 94 Range % 85-105 -
回收率：
The recovery of mouse KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 85-96 EDTA plasma (n=4) 100 95-103 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 100 2.513 2.569 2.541 2.349 50 2.163 2.168 2.166 1.974 25 1.633 1.658 1.646 1.454 12.5 0.992 0.999 0.996 0.804 6.25 0.562 0.571 0.567 0.375 3.12 0.368 0.383 0.376 0.184 1.56 0.272 0.291 0.282 0.090 0 0.190 0.193 0.192 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Hair Growth Promotion Activity and Its Mechanism of Polygonum multiflorum Yunfei Li. et al,Evidence-Based Complementary and Alternative Medicine,2015
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靶點(diǎn)詳情
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功能：Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
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基因功能參考文獻(xiàn)：
- Tregs express kgf. Treg-specific expression of kgf regulates alveolar epithelial proliferation during the resolution phase of acute lung injury and in a model of regenerative alveologenesis in vivo. AT2 cells cocultured with Tregs lacking kgf have decreased rates of proliferation. PMID: 28296468
- A role for endogenous KGF in maintenance of normal pulmonary innate immune function. PMID: 26919897
- Suppression of miR-219-5p may benefit the liver regeneration and prevent cirrhosis through increasing KGF. PMID: 27855391
- we examine how the two presynaptic organizers FGF22 and FGF7 are transported to the appropriate locations so that they act as synapse-type-specific organizing molecules. PMID: 25431136
- Exendin-4 increased fibroblast growth factor 7 (Fgf7) expression in colonic polyps of Apc(Min/+) mice. PMID: 25738454
- Our observations also point towards PI3K acting as a morphogenic switch for FGF7 signalling. PMID: 25460003
- Reverse transcriptase polymerase chain reaction studies showed that three members, Tgfa, Hbegf,and Nrg1 of the EGF family were expressed in the epithelium cultured with FGF7 + LPA as well as in the epithelium freshly isolated from the rudiments. PMID: 24227310
- IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID: 23582717
- FGF7 is downregulated in MED1-deficient mesenchymal cells. FGF7 produced by mesenchymal stromal cells is a novel hematopoietic niche molecule. PMID: 24051090
- KGF pretreatment could improve radiation-induced intestinal injury including the epithelial structure and function in a mouse model, as shown by Zo-1 protein and Claudin-1. PMID: 23464848
- KGF could up-regulate IL-7 expression through the STAT1/IRF-1, IRF-2 signaling pathway, which is a new insight in potential effects of KGF on the intestinal mucosal immune system. PMID: 23554911
- Fibroblast growth factor 7 (Fgf7) was identified as a target of miR-143 in murine adipogenesis. PMID: 23247565
- FGF7 is a critical regulator of liver progenitor cells PMID: 23322300
- Given the specifically targeted effects of FGF-7 on epithelial cells, the authors' findings suggest that further FGF-7-dependent epithelial-mesenchymal interaction exists that may be important in diabetic wound healing. PMID: 22094769
- Keratinocyte growth factor gene transduction ameliorates pulmonary fibrosis induced by bleomycin in mice PMID: 21131445
- These results suggest that KGF may play an important role in the regulation of VEGF gene expression and angiogenesis during wound healing. PMID: 21672345
- KGF promotes preadipocyte proliferation by an autocrine mechanism that involves activation of the PI3K/Akt signaling pathway PMID: 20069574
- two members of the fibroblast growth factor (FGF) family, FGF22 and FGF7, promote the organization of excitatory and inhibitory presynaptic terminals, respectively, as target-derived presynaptic organizers PMID: 20505669
- keratinocyte growth factor works via an inducible lentivirus to protect bone marrow cells against bleomycin-induced pulmonary fibrosis PMID: 19956603
- Protection from thymic epithelial cell injury by keratinocyte growth factor PMID: 12036893
- Keratinocyte growth factor preserves normal thymopoiesis and thymic microenvironment during experimental graft-versus-host disease. PMID: 12091365
- Following activation by KGF binding, KGF and the KGF receptor remain associated in active complexes through the endocytic pathway, which is described. PMID: 12122441
- Stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds. PMID: 12161023
- gammadelta cell-deficient mice (TCRdelta(-/-)) and KGF-deficient mice (KGF(-/-)) were more prone than wild-type mice to DSS-induced mucosal injury and demonstrated delayed tissue repair after termination of DSS treatment PMID: 12376619
- PAK4 interacts with KGF receptor and mediate anti-apoptosis effects of KGF on epithelial cells. PMID: 12529371
- In a mouse model of total parenteral nutrition (TPN), application of KGF to TPN mice reversed the decline of proliferation of EC during TPN and prevented the increase in EC apoptosis with TPN alone. PMID: 12592627
- KGF-induced Akt activation may play an important role in inhibiting lung alveolar cell death thereby preserving the lung architecture and function during oxidative stress PMID: 12732722
- BMP4 and FGF7/FGF10 arrest early T-cell development at the CD4-CD8-CD44+CD25- (double-negative 1 [DN1]) population and at the CD4-CD8- double-negative to CD4+CD8+ double-positive transition in a stromal compartment-dependent manner PMID: 12920023
- Structure-based mutational analyses in FGF7 identify new residues involved in specific interaction with FGFR2IIIb. PMID: 14527678
- KGF from gamma delta-T cell receptor-expressing intraepithelial lymphocytes is an important factor for maintenance of intestinal epithelial cell proliferation and villus growth. PMID: 15034027
- Biological activity of FGF-7 in RAG-1 mice indicates that immuno-dependent mechanisms are not required for the induction of urothelial cell proliferation by this growth factor. PMID: 15176316
- epithelial Wnt11, mesenchymal Gdnf and stromal Fgf7 signalling is coordinated by sprouty proteins during kidney development, which regulates ureteric branching PMID: 15201220
- KGF antibody abolished the growth promoting effect of GLP-2 in the large intestine, but not in the small intestine PMID: 15544847
- KGF appears to be an important growth factor for local resident progenitor epithelial cell repair PMID: 17332441
- FGF7 improved survival during acute lung injury in adult mouse lung after short-term expression, but paradoxically induced inflammation and injury after persistent expression. PMID: 17994369
- Krt12-rtTA/tet-O-FGF-7 mice may be a suitable animal model for the study of the molecular and cellular mechanisms of human ocular surface squamous neoplasia. PMID: 18276784
- Ectopic expression of KGF in acinar cells promotes the expansion of the beta-cell lineage in vivo through activation of the PKB/Akt pathway. PMID: 18372394
- p63/p51-induced onset of keratinocyte differentiation via the c-Jun N-terminal kinase pathway is counteracted by keratinocyte growth factor PMID: 18849344
- Fgf7 protects against acute lung injury PMID: 17994369
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亞細(xì)胞定位：Secreted.
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蛋白家族：Heparin-binding growth factors family
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