Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

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中文名稱：

小鼠胰島素樣肽INSL5(INSL5)酶聯(lián)免疫試劑盒
貨號：

CSB-EL011749MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠胰島素樣肽INSL5(INSL5)酶聯(lián)免疫試劑盒（CSB-EL011749MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的INSL5含量。INSL5，即胰島素樣肽5，是胰島素超家族的一員，具有胰島素家族的經(jīng)典結(jié)構(gòu)。其研究機(jī)制主要涉及與受體RXFP4的相互作用，調(diào)節(jié)能量代謝、食欲調(diào)節(jié)和腸道蠕動等生理功能。近期研究發(fā)現(xiàn)，INSL5在腫瘤糖代謝調(diào)控中發(fā)揮重要作用，通過激活STAT5信號通路促進(jìn)糖酵解相關(guān)基因的表達(dá)，影響細(xì)胞糖代謝重編程，進(jìn)而促進(jìn)腫瘤進(jìn)展。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，可為研究INSL5在代謝綜合征、食欲異常調(diào)控、胃腸道功能紊亂等領(lǐng)域的分子機(jī)制提供可靠工具，也可用于評估藥物干預(yù)或基因修飾模型中INSL5的表達(dá)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Insl5 ELISA Kit; Rif ELISA Kit; Rif2 ELISA Kit; Zins3 ELISA Kit; Insulin-like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Relaxin/insulin-like factor 2 ELISA Kit; Relaxin/insulin-like protein) [Cleaved into: Insulin-like peptide INSL5 B chain; Insulin-like peptide INSL5 A chain] ELISA Kit
縮寫：

INSL5
Uniprot No.：

Q9WUG6
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse INSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	90-96
1:200	Average %	100
	Range %	97-103
1:400	Average %	88
	Range %	85-93
1:800	Average %	107
	Range %	103-110

回收率：

The recovery of mouse INSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	98	93-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.417	2.446	2.432	2.262
100	2.046	2.008	2.027	1.857
50	1.562	1.568	1.565	1.395
25	1.017	1.035	1.026	0.856
12.5	0.692	0.669	0.681	0.511
6.25	0.425	0.435	0.430	0.260
3.12	0.306	0.330	0.318	0.148
0	0.169	0.171	0.170

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
基因功能參考文獻(xiàn)：
1. Mass spectral analysis of purified enteroendocrine cells and tissue homogenates identified the exact sequence of A and B chains of INSL5 endogenously expressed in L-cells. PMID: 28857318
2. Insl5 is an orexigenic hormone released from colonic L-cells, which promotes appetite during conditions of energy deprivation. PMID: 25028498
3. INSL5 may be a unique marker of colorectal enteroendocrine cells, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors. PMID: 23438439
4. Insl5 knockout mice displayed impaired male and female fertility that are due to marked reduction in sperm motility and irregular length of the estrous cycle. Also impaired glucose homeostasis due to glucose intolerance. PMID: 22822165
5. Insulin-like peptide 5 is expressed in mouse brain and has a role in mobilization of calcium PMID: 16601133
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Insulin family
組織特異性：

Highest expression in colon with lower levels in thymus. Minimal levels in testis.
數(shù)據(jù)庫鏈接：

KEGG: mmu:23919

STRING: 10090.ENSMUSP00000081416

UniGene: Mm.100163

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.