Mouse Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠XIIA組分泌型磷脂酶A2(PLA2G12A)酶聯(lián)免疫試劑盒（CSB-EL018086MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PLA2G12A含量。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，適用于科研實(shí)驗(yàn)中定量分析小鼠模型或細(xì)胞實(shí)驗(yàn)中PLA2G12A的動(dòng)態(tài)變化，可廣泛用于探索脂質(zhì)代謝疾病、動(dòng)脈粥樣硬化、非酒精性脂肪肝等疾病的分子機(jī)制，或評(píng)估藥物干預(yù)對(duì)PLA2G12A表達(dá)的影響，為相關(guān)基礎(chǔ)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Pla2g12a ELISA Kit; Pla2g12 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; GXII sPLA2 ELISA Kit; sPLA2-XII ELISA Kit; EC 3.1.1.4 ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit
縮寫：

PLA2G12A
Uniprot No.：

Q9EPR2
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	8-89
1:2	Average %	98
	Range %	94-103
1:4	Average %	85
	Range %	82-89
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of mouse PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	90	87-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
亞細(xì)胞定位：

Secreted. Cytoplasm.
蛋白家族：

Phospholipase A2 family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:66350

STRING: 10090.ENSMUSP00000029629

UniGene: Mm.151951