Mouse Galectin 3(GAL-3) ELISA Kit

Mouse Galectin 3(GAL-3) ELISA Kit

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中文名稱：

小鼠半乳糖凝集素-3(Gal-3)酶聯免疫試劑盒
貨號：

CSB-E14296m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠半乳糖凝集素-3(Gal-3)酶聯免疫試劑盒（CSB-E14296m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LGALS3含量。LGALS3 是一個重要靶點。它在細胞黏附、增殖、凋亡等多種生理病理過程中發揮作用。相關研究圍繞其與腫瘤發生發展、免疫調節等機制展開，探索通過抑制其活性來開發新型治療藥物，為癌癥等疾病的治療帶來新希望。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于小鼠疾病模型構建、藥物干預研究及分子機制探索等科研領域，特別針對心血管疾病、肝纖維化或腫瘤微環境研究中Gal - 3的動態變化監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
縮寫：

LGALS3
Uniprot No.：

P16110
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-103
1:2	Average %	90
	Range %	82-99
1:4	Average %	92
	Range %	86-100
1:8	Average %	92
	Range %	89-103

回收率：

The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	97	91-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.852	2.842	2.847	2.703
10	2.207	2.280	2.244	2.100
5	1.357	1.384	1.371	1.227
2.5	0.841	0.862	0.852	0.708
1.25	0.475	0.458	0.467	0.323
0.625	0.283	0.297	0.290	0.146
0.312	0.231	0.244	0.238	0.094
0	0.142	0.146	0.144

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Galectin-3 Inhibition Ameliorates Alveolar Epithelial Cell Pyroptosis in Phosgene-Induced Acute Lung Injury O Memet,/,/
Noni (Morinda citrifolia L.) Fruit Polysaccharide Attenuated Inflammatory Bowel Disease Mice: Association of STAT3/NLRP3 Signaling Pathways MY Jin,/,2023
Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization H Liu,iScience,2023
Evaluation of Annexin A7, Galectin-3 and Gelsolin as possible biomarkers of hepatocarcinoma lymphatic metastasis Wang X et al,Biomed Pharmacother,2014

功能：

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
亞細胞定位：

Cytoplasm. Nucleus. Secreted.
組織特異性：

The highest levels are found in activated macrophages.
數據庫鏈接：

STRING: 10090.ENSMUSP00000114350

UniGene: Mm.248615

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.