Mouse Fibulin-1(FBLN1) ELISA kit

Mouse Fibulin-1(FBLN1) ELISA kit

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中文名稱：

小鼠腓骨蛋白-1(FBLN1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL008452MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠腓骨蛋白-1(FBLN1)酶聯(lián)免疫試劑盒（CSB-EL008452MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FBLN1含量。FBLN1（fibulin-1）是一種分泌性糖蛋白，主要存在于細(xì)胞外基質(zhì)中，參與細(xì)胞粘附、遷移和信號(hào)傳導(dǎo)。其研究機(jī)制涉及與層粘連蛋白和巢蛋白的結(jié)合，以及通過鈣結(jié)合介導(dǎo)的血小板粘附。FBLN1的表達(dá)與多種疾病如動(dòng)脈粥樣硬化、癌癥等密切相關(guān)。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于研究FBLN1在纖維化疾病模型中的動(dòng)態(tài)變化、腫瘤微環(huán)境基質(zhì)重塑機(jī)制、胚胎發(fā)育相關(guān)信號(hào)通路調(diào)控等科研領(lǐng)域，支持體外實(shí)驗(yàn)中對(duì)不同樣本類型的FBLN1表達(dá)水平進(jìn)行精確量化分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Fbln1Fibulin-1 ELISA kit; FIBL-1 ELISA kit; Basement-membrane protein 90 ELISA kit; BM-90 ELISA kit
縮寫：

FBLN1
Uniprot No.：

Q08879
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	89-99
1:2000	Average %	95
	Range %	91-101
1:4000	Average %	94
	Range %	89-98
1:8000	Average %	98
	Range %	92-103

回收率：

The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-97
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.419	2.319	2.369	2.203
100	1.701	1.601	1.651	1.485
50	1.022	1.002	1.012	0.846
25	0.664	0.654	0.659	0.493
12.5	0.448	0.425	0.437	0.271
6.25	0.375	0.365	0.370	0.204
3.12	0.287	0.281	0.284	0.118
0	0.168	0.164	0.166

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.
基因功能參考文獻(xiàn)：
1. Fbln1c plays a key role in regulating airway remodelling and inflammation in chronic asthma. PMID: 28862768
2. The FBLN1/FGF8 interaction may also be involved in the survival of neural crest cell population during development. PMID: 27402846
3. the findings indicate that Fbln1 normally suppresses proximal OFT EMT preventing proximal cushion hypercellularity and blood cell accumulation. PMID: 25575930
4. Fbln1 is a new positive modulator of the formation of membranous bone and endochondral bone in the skull PMID: 25201465
5. fibulin-1 regulates versican-dependent events in ventricular morphogenesis by promoting ADAMTS1 cleavage of versican leading to suppression of trabecular cardiomyocyte proliferation mediated by the ErbB2-Map kinase pathway. PMID: 22183742
6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2 PMID: 11733994
7. structural and functional characterization of promoter PMID: 11829738
8. binding of HPV E6 protein to fibulin-1 PMID: 12200142
9. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
10. A murine placental overgrowth phenotype is associated with elevated Fbln1 transcript levels. PMID: 16338003
11. Fbln1 is required for the directed migration and survival of cranial neueral crest cells contributing to the development of pharyngeal glands, craniofacial skeleton, cranial nerves, aortic arch arteries, cardiac outflow tract and cephalic blood vessels. PMID: 18538758
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亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibulin family
組織特異性：

Detected in most organs (brain, heart, lung, spleen, liver and kidney). Neurons are the predominant source of production in the brain. Not expressed significantly by astrocytes or microglia.
數(shù)據(jù)庫鏈接：

KEGG: mmu:14114

STRING: 10090.ENSMUSP00000054583

UniGene: Mm.297992

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.