Mouse Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠肌酸激酶同工酶MM(CK-MM)酶聯(lián)免疫試劑盒（CSB-E14407m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的CK-MM含量。CK-MM即肌酸激酶同工酶，主要存在于骨骼肌和心肌中，在臨床上是診斷急性心肌梗死等疾病的重要指標(biāo)。研究機(jī)制方面，通過(guò)檢測(cè)血液中CK-MM的含量變化，可反映心肌或骨骼肌細(xì)胞損傷情況，在心肌梗死時(shí)其水平會(huì)快速升高。試劑盒檢測(cè)范圍為0.156 mU/mL-10 mU/mL，適用于基礎(chǔ)科研中骨骼肌損傷模型評(píng)估、運(yùn)動(dòng)生理代謝研究、肌肉相關(guān)疾病機(jī)制探索等場(chǎng)景，例如通過(guò)監(jiān)測(cè)血清CK-MM濃度變化研究肌肉再生能力或病理?yè)p傷程度。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

CK-MM
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.156 mU/mL-10 mU/mL
靈敏度：

0.039 mU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-98
1:2	Average %	98
	Range %	92-103
1:4	Average %	104
	Range %	98-108
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of mouse CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	100	95-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.