Mouse Complement fragment 5a,C5a ELISA Kit

Mouse Complement fragment 5a,C5a ELISA Kit

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中文名稱：

小鼠補體片斷5a(C5a)酶聯(lián)免疫試劑盒
貨號：

CSB-E08514m
規(guī)格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠補體片斷5a(C5a)酶聯(lián)免疫試劑盒（CSB-E08514m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是補體系統(tǒng)激活過程中產(chǎn)生的重要炎癥介質(zhì)。它在免疫調(diào)節(jié)、炎癥反應等方面發(fā)揮關(guān)鍵作用。研究機制主要聚焦于其與受體結(jié)合后引發(fā)的細胞信號傳導通路，如激活免疫細胞、促進趨化等，可作為炎癥相關(guān)疾病治療的潛在靶點。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，靈敏度為9.75 pg/mL。適用于體外實驗中對炎癥模型、免疫調(diào)控機制或藥物干預效果的評估，為研究補體系統(tǒng)相關(guān)疾病的分子機制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C5a
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	85
	Range %	81-90
1:40	Average %	98
	Range %	91-105
1:80	Average %	89
	Range %	84-94
1:160	Average %	90
	Range %	86-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2500

2.844

2.734

2.789

2.662

1250

2.331

2.197

2.264

2.137

625

1.488

1.528

1.508

1.381

312.5

0.810

0.829

0.819

0.693

156.25

0.546

0.521

0.533

0.407

0.357

0.363

0.360

0.234

0.221

0.214

0.218

0.091

0.128

0.125

0.127

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
Blockade of C5aR1 alleviates liver inflammation and fibrosis in a mouse model of NASH by regulating TLR4 signaling and macrophage polarization K Jiang,Journal of gastroenterology,2023
Cryogel/hydrogel biomaterials and acupuncture combined to promote diabetic skin wound healing through immunomodulation TY Chen,Biomaterials,2020
Heparin-like surface modification of polyethersulfone membrane and its biocompatibility Min Tang et al,Journal of Colloid and Interface Science,2012

最新研究進展：

C5a，全稱為第五組分激活肽（complement component 5a），是補體系統(tǒng)中的一種小分子肽類分子。當補體系統(tǒng)被激活后，C5分子會被切割成C5a和C5b兩部分，C5a是其中的一個切割產(chǎn)物。C5a是一個強烈的炎癥介質(zhì)，可以吸引和激活免疫細胞，如嗜中性粒細胞、單核細胞和巨噬細胞，促進炎癥反應的形成。C5a的異常高水平與許多炎癥性疾病有關(guān)，例如風濕性關(guān)節(jié)炎、肺炎、系統(tǒng)性紅斑狼瘡等。

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.