Mouse C-Reactive Protein,CRP ELISA Kit
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中文名稱：
小鼠C反應蛋白(CRP)酶聯免疫試劑盒
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貨號：
CSB-E07923m
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規格：
96T/48T
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價格：
￥3800/￥2500
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其他：
產品詳情
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產品描述：
小鼠C反應蛋白(CRP)酶聯免疫試劑盒（CSB-E07923m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CRP含量。CRP即C反應蛋白，是一種急性時相反應蛋白。在炎癥、感染、組織損傷等情況下其水平會迅速升高。研究機制方面，它可與病原體結合，激活補體系統，在免疫防御中發揮作用，也是臨床常用的炎癥和疾病活動監測指標。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，常用于評估機體免疫狀態和炎癥進程；特別適用于實驗動物模型中炎癥相關疾病研究、藥物抗炎效果評價以及免疫調節機制探索等科研領域；可為心血管疾病、代謝綜合征、感染性疾病等基礎研究提供可靠的實驗數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
Crp ELISA Kit; Ptx1C-reactive protein ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Mus musculus (Mouse)
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樣本類型：
serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：
15.6 ng/mL-1000 ng/mL
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靈敏度：
3.9 ng/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Immunology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:20 | Average % | 90 | |
| Range % | 86-93 | |
| 1:40 | Average % | 103 | |
| Range % | 99-106 | |
| 1:80 | Average % | 93 | |
| Range % | 87-96 | |
| 1:160 | Average % | 98 | |
| Range % | 93-101 | |
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回收率：
| The recovery of mouse CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-97 | |
| EDTA plasma (n=4) | 97 | 90-100 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.206 | 2.124 | 2.165 | 1.995 | | | 500 | 1.661 | 1.655 | 1.658 | 1.488 | | | 250 | 1.138 | 1.122 | 1.130 | 0.960 | | | 125 | 0.727 | 0.721 | 0.724 | 0.554 | | | 62.5 | 0.424 | 0.435 | 0.430 | 0.260 | | | 31.2 | 0.292 | 0.305 | 0.299 | 0.129 | | | 15.6 | 0.228 | 0.234 | 0.231 | 0.061 | | | 0 | 0.168 | 0.172 | 0.170 | | |
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本試劑盒所含材料：
- An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
- Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
- Biotin-labeled CRPantibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
- Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
- HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
- Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
- TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Seal the microtiter plate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Renoprotective Effects of Jaeumgeonhwa-tang and Gunryeong-tang in a Renal Ischemia-Reperfusion Injury Model via Regulation of Inflammation and Oxidative Stress
YP Lee, MH Hong, YJ Yang, A Tai, CY Yoo,Herbal Formula Science,2025
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Therapeutic anti-inflammatory immune potentials of some seaweeds extracts on chemically induced liver injury in mice
E Bases, MM El-Sheekh, SM El Shafay,Scientific Reports,2025
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Live and Heat-Inactivated Streptococcus thermophilus MN-ZLW-002 Mediate the Gut–Brain Axis, Alleviating Cognitive Dysfunction in APP/PS1 Mice
Y Zhang,Nutrients,2024
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Epigallocatechin-3-gallate Enhances Cognitive and Memory Performance and Protects Against Brain Injury in Methionine-induced Hyperhomocysteinemia Through Interdependent Molecular Pathways
MD Mostafa,Neurotoxicity research,2022
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Lectin-like oxidized low-density lipoprotein receptor-1 attenuates pneumonia-induced lung injury
FT Korkmaz,JCI Insight,2022
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Low Vitamin D Status Is Associated with Inflammation in Patients with Chronic Obstructive Pulmonary Disease
L Fu,The Journal of Immunology,2020
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Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes
M Wu,Journal of Advanced Research,2020
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Novel Treatment of Experimental Autoimmune Prostatitis by Nanoparticle-Conjugated Autoantigen Peptide T2
Cheng Y, et al,Inflammation,2019
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HIF1α deletion facilitates adipose stem cells to repair renal fibrosis in diabetic mice
Tang Q.et al,In Vitro Cell Dev Biol Anim,2018
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6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice
Xu Y.et al,Biomed Pharmacother,2017
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Does a Treadmill Running Exercise Contribute to Preventing Deterioration of Bone Mineral Density and Bone Quality of the Femur in KK-Ay Mice, a Type 2 Diabetic Animal Model?
Takagi S.et al,Calcif Tissue Int. ,2017
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DNA vaccine encoding the major virulence factors of Shiga toxin type 2e (Stx2e)-expressing Escherichia coli induces protection in mice
Ren W et al,Vaccine,2012
產品評價
樣品類型：血清
樣品信息：小鼠
稀釋比：1:100
產品評價： 我用CSB-E07923m檢測炎癥血清，樣本的OD值0.8-0.9，測了不同組，結果較為準確，標品操作手冊清楚明了。
樣品類型：血清
樣品信息：小鼠
稀釋比：1:100
產品評價： 我用CSB-E07923m測關節炎小鼠血清OD值在0.72左右，標準曲線為y=0.00115x+0.10743,r2=0.99309,結果精準靈敏
樣品類型：血清
樣品信息：小鼠
稀釋比：其他 1:10
產品評價： 我用CSB-E07923m檢測正常血清，標準曲線OD值為0.105，0.176，0.243，0.398，0.59，0.965，1.438，2.096，樣本的OD值為0.7-1.8，產品的靈敏度和容錯率都很高，操作有點失誤也能測出較好的值
靶點詳情
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功能：
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
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基因功能參考文獻：
- CRP is pathogenic in type-2 diabetes (T2DN). CRP may promote CD32b- NF-kappaB signaling to mediate renal inflammation; whereas, CRP may enhance renal fibrosis in T2DN via CD32b-Smad3-mTOR signaling. PMID: 27221338
- FcgammaRIIb and FcgammaRIII are involved in the CRP-induced expression of MMPs and TIMP-1 and the CRP-induced phosphorylation of p38, whereas the FcgammaR-independent pathway may regulate the CRP-induced MMP-11 expression and the CRP-induced ERK1/2 phosphorylation. PMID: 28539825
- C-reactive protein may promote acute kidney injury by impairing tubular epithelial cell regeneration via the CD32-Smad3-p27-driven inhibition of the CDK2/cyclin E complex. PMID: 27470679
- These results suggested that CRP/oxidized low density lipoprotein/beta 2-Glycoprotein I complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFkappaB signaling pathways. PMID: 27512978
- these data unexpectedly identify CBS as the major recognition site of mCRP and suggest that this motif may be exploited to tune the proinflammatory actions of mCRP. PMID: 26907682
- CRP decreased adiponectin expression and multimerization, while CRP-induced decline in adiponectin might be mediated through the PI3K/Akt pathway. PMID: 26812237
- mCRP may be responsible for promoting dementia after ischaemia and mCRP clearance could inform therapeutic avenues to reduce the risk of future dementia. PMID: 26335098
- Effects of C-Reactive Protein on the Cardiac Differentiation of Mouse Embryonic Stem Cells PMID: 26328491
- These data indicated that CRP may have a direct role on osteoclast and osteoblast differentiation via toll-like receptor signaling pathways. PMID: 26687448
- Data suggest that serum amyloid P (SAP) activates CD209 DC-SIGN to regulate the innate immune system differently from C-reactive protein (CRP), and that DC-SIGN is a target for antifibrotics. PMID: 26106150
- the acute phase protein C-reactive protein (CRP), a ligand for Fc receptors on phagocytes, enhances antibody-mediated platelet destruction by phagocytes in vivo PMID: 25548320
- CRP has therapeutic potential against cancer through decreasing the accumulation of M2 macrophages and angiogenesis within tumors. PMID: 25088786
- Orphan nuclear receptor Errgamma induces C-reactive protein gene expression through induction of ER-bound Bzip transmembrane transcription factor CREBH. PMID: 24466039
- The interactions of CRP and the BBB are a two-phase process, with increased paracellular permeability at a high dose that enables its entry into the CNS and serves to induce reactive gliosis and impair CNS function. PMID: 23018453
- In mice with photochemically induced thrombosis, acrolein produced at the locus of infarction increased the level of IL-6 and then CRP in plasma. PMID: 23763486
- CRP directly inhibits tumoral lymphangiogenesis and lymph node metastasis in mice. PMID: 23859309
- Which processes in glucose homeostasis are altered by CRP? Data suggest that CRP attenuates insulin-induced skeletal muscle blood flow; CRP contributes to insulin resistance via IgG receptor IIB-mediated inhibition of glucose delivery to muscle. PMID: 23069625
- CRP is not only a biomarker, but also a mediator in DKD. PMID: 21744073
- C-reactive protein promotes acute renal inflammation and fibrosis in unilateral ureteral obstructive nephropathy in mice. PMID: 21383672
- These findings reveal a novel nutrient-dependent interaction between Sirt1 and HNF-1alpha which results in regulation of Crp expression. PMID: 21176092
- Studies show that CRP itself may have a pathogenic role in the development of pressure overload-induced cardiac remodeling, possibly through enhanced inflammation and oxidative stress. PMID: 21220701
- No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice. PMID: 21149301
- Modified and natural C-reactive protein have opposite effects on atherosclerosis in apolipoprotein E-deficient mice. PMID: 16087790
- the contribution of CRP's ability of activating the classical pathway of complement in the protection of mice from lethal infection with virulent Streptococcus pneumoniae type 3 PMID: 16547275
- nCRP can facilitate cardiovascular disease through impairment of endothelium-dependent vasoreactivity, in a manner that involves increased iNOS activity and a potential for increased peroxynitrite formation. PMID: 17669410
- the response to vascular injury provoked by human C-Reactive Protein in ovariectomized C-reactive protein transgenic mice depends on Fc gamma RI and probably requires its expression by F4/80+ cells PMID: 18063701
- In vivo analyses revealed significant increases in TF expression and decreases in TFPI expression in carotid arteries of CRP-Tg mice versus WT mice. PMID: 18276908
- CRP plays a crucial role in the induction, amplification, and prolongation of inflammatory processes, including atherosclerotic lesions PMID: 18402807
- C-reactive protein has a role in impaired endothelial function PMID: 18433756
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亞細胞定位：
Secreted.
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蛋白家族：
Pentraxin family
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組織特異性：
Found in plasma.
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數據庫鏈接：
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