Mouse Apolipoprotein B48 (apo-B48) ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠載脂蛋白B48(apo-B48)酶聯免疫試劑盒（CSB-E16506m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的apo-B48含量。apo-B48是重要的血脂相關靶點。其背景在于它是乳糜微粒的主要載脂蛋白，在脂質代謝中起關鍵作用。研究機制主要圍繞調控其合成、分泌等環節，以降低血漿中富含甘油三酯脂蛋白水平，為高脂血癥等疾病治療提供新方向。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于研究脂代謝相關動物模型中apo - B48的動態變化，例如高脂飲食誘導的肥胖小鼠、腸道功能缺陷模型或基因編輯動物；為探究脂蛋白代謝調控機制、藥物干預效果評估等基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

apo-B48
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 ng/mL-4000 ng/mL
靈敏度：

15.6 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-B48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	96
	Range %	92-104
1:400	Average %	93
	Range %	89-97
1:800	Average %	87
	Range %	84-92
1:1600	Average %	87
	Range %	82-93

回收率：

The recovery of mouse apo-B48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	98	94-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.