Mouse Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody) ELISA kit

產品詳情

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文獻引用 (6) 評價 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠抗環胍氨酸肽抗體(Anti-CCP-antibody)酶聯免疫試劑盒（CSB-EQ027743MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Anti-CCP-Ab含量。Anti - CCP - Ab即抗環瓜氨酸肽抗體，它是類風濕關節炎高度特異的血清學標志物。其研究機制主要是能識別環化修飾的抗原決定簇，與類風濕關節炎發病機制中的自身免疫反應相關，檢測它有助于類風濕關節炎的早期診斷及病情評估。試劑盒檢測范圍為6.25 U/mL-400 U/mL，適用于基礎科研中疾病機制探索、藥物研發階段的藥效學評價以及動物模型中的生物標志物動態監測等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Anti-CCP-Ab
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

6.25 U/mL-400 U/mL
靈敏度：

1.56 U/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-101
1:2	Average %	97
	Range %	91-106
1:4	Average %	95
	Range %	90-106
1:8	Average %	96
	Range %	89-104

回收率：

The recovery of mouse anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-110
EDTA plasma (n=4)	95	83-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 2 個評價

樣品類型：Serum

樣品信息：小鼠

稀釋比：其他

產品評價：我用anti-CCP試劑盒檢測小鼠正常、模型、給藥組的血清樣本，數據比較穩定，組內差異小。個別樣本復測值差別也很小，試劑盒質量穩定。以前買的試劑盒剩余了幾個孔，大概過了半年多再用，還是很不錯，贊！希望華美繼續保持！

By 呂老師

樣品類型：Serum

樣品信息：小鼠

稀釋比：其他

產品評價：試劑盒質量很好，測試很穩定，標曲也很好。

By 賓老師