Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

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中文名稱：

小鼠血管生成素相關蛋白6(ANGPTL6)酶聯免疫試劑盒
貨號：

CSB-EL001714MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠血管生成素相關蛋白6(ANGPTL6)酶聯免疫試劑盒（CSB-EL001714MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ANGPTL6含量。ANGPTL6，也稱為Angiopoietin-like 6，是一種與血管生成和細胞分化相關的蛋白質編碼基因。其研究顯示，ANGPTL6基因突變與家族性顱內動脈瘤形成有關，且在冠心病患者中，ANGPTL6水平與冠狀動脈病變嚴重程度呈負相關，提示其可能作為潛在的治療靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于基礎科研中探索ANGPTL6在代謝性疾病模型、血管生成機制或相關信號通路中的表達變化，為體外實驗提供高效定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Angptl6 ELISA kit; AgfAngiopoietin-related protein 6 ELISA kit; Angiopoietin-like protein 6 ELISA kit; Angiopoietin-related growth factor ELISA kit
縮寫：

ANGPTL6
Uniprot No.：

Q8R0Z6
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-96
1:200	Average %	98
	Range %	93-102
1:400	Average %	99
	Range %	95-107
1:800	Average %	91
	Range %	87-95

回收率：

The recovery of mouse ANGPTL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	99	96-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.616	2.793	2.705	2.546
2000	1.927	2.036	1.982	1.823
1000	1.287	1.388	1.338	1.179
500	0.769	0.729	0.749	0.590
250	0.577	0.604	0.591	0.432
125	0.394	0.401	0.398	0.239
62.5	0.247	0.267	0.257	0.098
0	0.164	0.154	0.159

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Leptin regulates the expression of angiopoietin-like 6 Min-Jeong Kim.et al,Biochemical and Biophysical Research Communications,2018
ANGPTL6 expression is coupled with mitochondrial OXPHOS function to regulate adipose FGF21 Seul Gi Kang.et al,Endocrinology.,2017

功能：

May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure.
基因功能參考文獻：
1. Since upregulation of ANGPTL6 is induced on metabolic stress, we investigated the hepatic expression of ANGPTL6 by leptin, a representative adipokine of obesity. Mice on a high-fat diet showed increased serum leptin levels and hepatic Angptl6 expression. PMID: 29852166
2. Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. PMID: 27698489
3. mitochondrial OXPHOS function governs the expression of ANGPTL6, which is an essential factor for FGF21 production in adipose tissue and cultured adipocytes. PMID: 28184000
4. In addition to pronounced angiogenesis and epidermal hyperplasia, marked enlargement of dermal lymphatic vessels was observed in UV-B-exposed Angptl6/AGF transgenic mice. PMID: 22132739
5. we show that (>80%) of the AGF-deficient mice die at about embryonic day 13, while the surviving AGF-deficient mice develop marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure PMID: 15778720
6. Our results strongly suggest that AGF is an integrin ligand as well as a mitogenic growth factor and theoretically participates in cutaneous wound healing in a more complex mechanism. PMID: 16806062
7. Activation of an ERK1/2-eNOS-NO pathway is a crucial signaling mechanism by which angiopoietin-related growth factor increases blood flow through induction of angiogenesis and arteriogenesis. PMID: 18258819
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亞細胞定位：

Secreted.
組織特異性：

Highly expressed in the liver, specifically in hepatocytes, and weakly in the heart. Expressed in hematopoietic cells, platelets and mast cells, and detected at wounded skin.
數據庫鏈接：

KEGG: mmu:70726

STRING: 10090.ENSMUSP00000035784

UniGene: Mm.34019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.