Mouse 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠6酮前列腺素F1a(6-keto-PGF1a)酶聯(lián)免疫試劑盒（CSB-E09422m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的6ketoPGF1a含量。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，適用于心血管疾病模型、抗血栓藥物研究、組織缺血再灌注損傷等科研場景的實(shí)驗(yàn)分析，為血管活性物質(zhì)研究及藥效評價(jià)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

6ketoPGF1a
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 pg/mL-1000 pg/mL
靈敏度：

25 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-109
1:2	Average %	92
	Range %	86-105
1:4	Average %	90
	Range %	85-105
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of mouse 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-107
EDTA plasma (n=4)	94	83-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.