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中文名稱：人色氨酰tRNA合成酶(WARS)酶聯免疫試劑盒
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貨號：CSB-E11789h
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規格：96T/48T
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價格：￥3200/￥2500
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其他：
產品詳情
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產品描述：人色氨酰tRNA合成酶(WARS)酶聯免疫試劑盒（CSB-E11789h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細胞裂解物、組織勻漿樣本中的WARS含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于基礎科研領域中對細胞應激機制、信號通路調控或疾病模型（如炎癥、腫瘤等）中WARS蛋白表達水平變化的定量研究，為探索該酶在細胞生物學過程中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Gamma 2 ELISA Kit; GAMMA-2 ELISA Kit; Gamma2 ELISA Kit; hWRS ELISA Kit; IFI 53 ELISA Kit; IFI53 ELISA Kit; IFP 53 ELISA Kit; IFP53 ELISA Kit; Interferon induced protein 53 ELISA Kit; Interferon-induced protein 53 ELISA Kit; SYWC_HUMAN ELISA Kit; T2-TrpRS ELISA Kit; TrpRS ELISA Kit; Tryptophan tRNA ligase; cytoplasmic ELISA Kit; Tryptophan tRNA ligase 1 cytoplasmic ELISA Kit; Tryptophan tRNA ligase ELISA Kit; Tryptophan--tRNA ligase ELISA Kit; Tryptophanyl tRNA synthetase ELISA Kit; Tryptophanyl tRNA synthetase cytoplasmic ELISA Kit; WARS ELISA Kit; WARS protein ELISA Kit; WRS ELISA Kit
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縮寫：WARS
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, cell lysates, tissue homogenates
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檢測范圍：0.312 ng/mL-20 ng/mL
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靈敏度：0.078 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Epigenetics and Nuclear Signaling
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human WARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 88-95 1:2 Average % 87 Range % 81-93 1:4 Average % 97 Range % 93-101 1:8 Average % 100 Range % 94-106 -
回收率：
The recovery of human WARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 84-95 EDTA plasma (n=4) 93 88-99 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 1.972 2.050 2.011 1.920 10 1.446 1.556 1.501 1.410 5 1.027 1.101 1.064 0.973 2.5 0.638 0.606 0.622 0.531 1.25 0.381 0.387 0.384 0.293 0.625 0.293 0.275 0.284 0.193 0.312 0.195 0.201 0.198 0.107 0 0.093 0.089 0.091 -
數據處理：
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貨期：3-5 working days
引用文獻
- Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism Ara煤jo JR,Cell Host Microbe,2020
- Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer Suh JH,J Clin Med,2020
- Serum levels and activity of indoleamine2, 3-dioxygenase and tryptophanyl-tRNA synthetase and their association with disease severity in patients with chronic kidney Bao YS et al,Biomarkers,2013
產品評價
相關產品
靶點詳情
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功能：Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
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基因功能參考文獻：
- Based on these results, secretion of full-length tryptophanyl-tRNA synthetase appears to work as a primary defence system against infection, acting before full activation of innate immunity. PMID: 27748732
- findings establish WARS as a gene whose mutations may cause distal hereditary motor neuropathy and alter canonical and non-canonical functions of tryptophanyl-tRNA synthetase. PMID: 28369220
- Overexpression of WARS predicts no recurrence and good survival for triple-negative breast cancer patients. PMID: 26209610
- Tryptophanyl-tRNA synthetase expression is up-regulated in patients with rheumatoid arthritis. PMID: 24515434
- Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14) PMID: 23670221
- Indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase may play critical roles in the immune pathogenesis of chronic kidney disease. PMID: 23651343
- Naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. PMID: 21442253
- Tryptophanyl-tRNA synthetase down-regulation by hypoxia may be a factor responsible for low TrpRS in pancreatic tumors with high metastatic ability. PMID: 21926542
- Mini-tryptophanyl-tRNA synthetase inhibited ischemic angiogenesis in rats. PMID: 20963594
- Tryptophanyl-tRNA synthetase is a multidomain protein exhibiting excellent allosteric communication, and this research has provided valuable structural as well as functional insights into the protein. PMID: 19768679
- Low tryptophanyl-tRNA synthetase is associated with recurrence in colorectal cancer. PMID: 19900940
- In this study, we show that a recombinant form of a COOH-terminal fragment of TrpRS is a potent antagonist of vascular endothelial growth factor-induced angiogenesis in a mouse model and of naturally occurring retinal angiogenesis in the neonatal mouse PMID: 11773625
- Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS PMID: 11773626
- Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains PMID: 11834741
- The recently discovered antiangiogenic and cell-signaling activities of tryptophanyl-tRNA synthetase bioactive fragments are discussed in this review. PMID: 12416978
- TrpRS may have a role in the maintenance of vascular homeostasis PMID: 14630953
- results suggest that mammalian and bacterial tryptophanyl-tRNA synthetase might use different mechanisms to recognize the substrate and modeling studies indicate that transfer RNA binds with the dimeric enzyme PMID: 14660560
- A crystal structure of human tryptophanyl-tRNA synthetase was solved at 2.1 A with a tryptophanyl-adenylate bound at the active site PMID: 14671330
- May play an important role in the intracellular regulation of protein synthesis under conditions of oxidative stress. PMID: 15628863
- These crystals captured two conformations of the human tryptophanyl-tRNA synthetase and tRNATrp complex, which are nearly identical with respect to the protein and a bound tryptophan. PMID: 16724112
- The first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. PMID: 16798914
- Results provide the first evidence of the involvement of heme in regulation of TrpRS aminoacylation activity. PMID: 17877375
- the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS. PMID: 17999956
- Analysis of the molecular basis of the mechanisms of the substrate recognition and the activation reaction by tryptophanyl-tRNA synthetase. PMID: 18180246
- Indoleamine 2,3-dioxygenase (IDO)-expression in antigen-presenting cells (APCs) may control autoimmune responses by depleting the available tryptophan, whereas tryptophanyl-tRNA synthetase (TTS) may counteract this effect PMID: 19363598
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亞細胞定位：Cytoplasm.
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蛋白家族：Class-I aminoacyl-tRNA synthetase family
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