Human telomerase,TE ELISA Kit
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中文名稱：人端粒酶(TE)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E08021h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人端粒酶(TE)酶聯(lián)免疫試劑盒（CSB-E08021h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的TE含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，科研人員可通過分析疾病模型動(dòng)物或體外培養(yǎng)細(xì)胞中的端粒酶表達(dá)動(dòng)態(tài)，探索腫瘤發(fā)生機(jī)制、評(píng)估抗衰老藥物對(duì)端粒酶活性的調(diào)控作用，或利用尿液樣本開展無創(chuàng)性研究。其寬泛的樣本兼容性為組織水平研究、細(xì)胞實(shí)驗(yàn)數(shù)據(jù)驗(yàn)證及生物標(biāo)志物篩選提供了可靠工具，適用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及分子生物學(xué)領(lǐng)域的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：N/A
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縮寫：TE
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種屬：Homo sapiens (Human)
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樣本類型：serum, cell culture supernates, urine, tissue homogenates
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檢測(cè)范圍：0.312 ng/mL-20 ng/mL
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靈敏度：0.078 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Epigenetics and Nuclear Signaling
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 80-96 1:2 Average % 99 Range % 91-105 1:4 Average % 94 Range % 92-98 1:8 Average % 89 Range % 86-100 -
回收率：
The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 80-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 2.630 2.501 2.566 2.464 10 2.161 2.062 2.112 2.010 5 1.602 1.541 1.572 1.470 2.5 0.931 0.898 0.915 0.813 1.25 0.532 0.501 0.517 0.415 0.625 0.322 0.314 0.318 0.216 0.312 0.241 0.227 0.234 0.132 0 0.105 0.099 0.102 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- The Influence of Metabolic Syndrome on Potential Aging Biomarkers in Participants with Metabolic Syndrome Compared to Healthy Controls I Cattaneo,Biomedicines,2024
- Evaluation of potential aging biomarkers in healthy individuals: telomerase, AGEs, GDF11/15, sirtuin 1, NAD+, NLRP3, DNA/RNA damage, and klotho P Borsky,Biogerontology,2023
- ATR-IR Spectroscopy Application to Diagnostic Screening of Advanced Endometriosis I Kokot,Oxidative medicine and cellular longevity,2022
- Potential Antiaging Effects of DLBS1649, a Centella asiatica Bioactive Extract AH Karsono,Journal of Experimental Pharmacology,2021
- Combination of HDE and BIIB021 efficiently inhibits cell proliferation and induces apoptosis via downregulating hTERT in myelodysplastic syndromes Shengyun Lin,Experimental and Therapeutic Medicine,2021
- The effect of 12-week resistance exercise training on serum levels of cellular aging process parameters in elderly men B Hooshmand-Moghadam,Experimental Gerontology,2020
- Telomerase level increase is related to n-3 polyunsaturated fatty acid efficacy in first episode schizophrenia: Secondary outcome analysis of the OFFER randomized Paweczyk T.et al,Prog Neuropsychopharmacol Biol Psychiatry,2017
產(chǎn)品評(píng)價(jià)
平均分:
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樣品類型：蛋白（重組/純化） 細(xì)胞裂解后的胞漿蛋白
樣品信息：人
稀釋比：其他 1:2
產(chǎn)品評(píng)價(jià)： 我用CSB-E08021h測(cè)量癌細(xì)胞裂解液中的端粒酶含量，稀釋一倍，OD在0.3左右。標(biāo)曲正常，端粒酶定量也符合文獻(xiàn)報(bào)道。使用方便，如果可以的話盒子做小一點(diǎn)，更好保存。
By 黃老師
相關(guān)產(chǎn)品
相關(guān)問答
■ 常見問題解答
Q:
We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.
A:
Thanks for your inquiry.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.
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