Human soluble amyloid precursor protein α (sAPPα) ELISA kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人可溶性淀粉酶前體蛋白α(sAPPα)酶聯免疫試劑盒（CSB-EQ027464HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液、腦脊液（CSF）樣本中的sAPPα含量。sAPPα是一個備受關注的靶點。其它在生理過程中有重要作用。研究機制上，目前認為其與神經系統等功能相關，參與神經保護、突觸可塑性調節等過程，深入探索其作用機制有望為相關神經疾病治療帶來新突破。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于神經科學領域的基礎研究，包括疾病模型中的蛋白代謝分析、細胞實驗中的分泌動態監測，以及藥物干預下sAPPα水平變化的評估等科研場景，為探索神經系統疾病機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

soluble amyloid precursor protein alpha, sAPP alpha
縮寫：

sAPPα
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF)
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sAPPα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-94
1:2	Average %	101
	Range %	90-105
1:4	Average %	91
	Range %	85-94
1:8	Average %	93
	Range %	85-97

回收率：

The recovery of human sAPPα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-97
EDTA plasma (n=4)	97	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.