Human phosphorylated microtubule-associated protein tau (pMAPT /pTAU) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人磷酸化Tau蛋白酶聯(lián)免疫試劑盒（CSB-E17929h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腦脊液（CSF）樣本中的pMAPT /pTAU含量。pMAPT/pTAU作為靶點，在神經(jīng)退行性疾病如阿爾茨海默病背景下備受關(guān)注。它與微管相關(guān)蛋白tau異常磷酸化有關(guān)。研究機制圍繞探索其磷酸化調(diào)控過程、異常磷酸化后對神經(jīng)細胞功能影響，期望以此為突破點開發(fā)疾病治療手段。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于神經(jīng)退行性疾病機制研究、磷酸化Tau蛋白動態(tài)變化監(jiān)測、藥物干預(yù)效果評估等科研領(lǐng)域，為探索病理過程中生物標志物變化提供可靠工具，尤其適用于阿爾茨海默病模型研究、體液生物標志物篩查及神經(jīng)科學(xué)相關(guān)基礎(chǔ)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

pMAPT /pTAU
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cerebrospinal fluid (CSF)
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pMAPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	87
	Range %	81-92
1:4	Average %	95
	Range %	90-99
1:8	Average %	101
	Range %	96-108

回收率：

The recovery of human pMAPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	98	93-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.