Human leukotriene E4,LT-E4 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人白三烯E4(LTE4)酶聯(lián)免疫試劑盒（CSB-E05176h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LT-E4含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于炎癥機(jī)制研究、藥物篩選評(píng)估或疾病模型（如哮喘、過敏性鼻炎）中的生物標(biāo)志物檢測；科研人員可通過該產(chǎn)品探究LTE4在炎癥信號(hào)通路中的作用，或評(píng)估抗炎藥物對(duì)白三烯代謝的調(diào)控效果，為呼吸道疾病及免疫相關(guān)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LT-E4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LT-E4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	95
	Range %	88-100
1:8	Average %	98
	Range %	91-103

回收率：

The recovery of human LT-E4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-93
EDTA plasma (n=4)	96	93-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.