Human glucocorticoid receptor-β,GR-β ELISA Kit

說明書下載

中文名稱：

人糖皮質(zhì)激素受體β(GR-β)酶聯(lián)免疫試劑盒
貨號：

CSB-E08889h
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)問答

產(chǎn)品詳情

產(chǎn)品描述：

人糖皮質(zhì)激素受體β(GR-β)酶聯(lián)免疫試劑盒（CSB-E08889h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的GR-β含量。GR-β即糖皮質(zhì)激素受體β，是核受體超家族成員。它能拮抗GR-α轉(zhuǎn)錄活性，影響糖皮質(zhì)激素效應(yīng)。研究發(fā)現(xiàn)其在哮喘等炎癥性疾病中異常表達，深入探究其作用機制，可為相關(guān)疾病的治療提供新的靶點與思路。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于科研領(lǐng)域?qū)R-β表達水平的精準(zhǔn)分析；可為體外研究GR-β在疾病模型中的表達調(diào)控、藥物干預(yù)效果評估以及信號通路機制探索提供可靠工具；實驗樣本兼容性廣泛，支持從基礎(chǔ)細(xì)胞實驗到動物組織研究的多樣化需求，操作流程標(biāo)準(zhǔn)化，適用于實驗室常規(guī)蛋白定量分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
縮寫：

GR-β
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.75 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	91
	Range %	88-95
1:8	Average %	89
	Range %	85-96

回收率：

The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	87	83-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.441	2.382	2.412	2.301
1250	1.692	1.725	1.709	1.598
625	1.021	1.103	1.062	0.951
312.5	0.563	0.539	0.551	0.440
156	0.327	0.314	0.321	0.210
78	0.245	0.258	0.252	0.141
39	0.185	0.191	0.188	0.077
0	0.108	0.113	0.111

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Human glucocorticoid receptor-β,GR-β ELISA Kit

產(chǎn)品詳情

產(chǎn)品評價

相關(guān)問答

Most popular with customers

產(chǎn)品中心

信號通路

技術(shù)信息

聯(lián)系信息