Human fetal hemoglobin,HBF ELISA Kit

Human fetal hemoglobin,HBF ELISA Kit

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中文名稱：

人胎兒血紅蛋白(HBF)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09598h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人胎兒血紅蛋白(HBF)酶聯(lián)免疫試劑盒（CSB-E09598h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、RBC裂解物樣本中的HBF含量。HBF（胎兒血紅蛋白）具有重要背景。它在胎兒發(fā)育階段是主要血紅蛋白，出生后逐漸被成人血紅蛋白替代。研究發(fā)現(xiàn)，提升 HBF 水平可改善某些血紅蛋白病。其研究機(jī)制聚焦于調(diào)控 HBF 表達(dá)的基因與信號(hào)通路，以探索治療相關(guān)疾病的新策略。試劑盒檢測(cè)范圍為125 ng/mL-2000 ng/mL，該產(chǎn)品適用于科研領(lǐng)域?qū)μ貉t蛋白表達(dá)調(diào)控機(jī)制的研究，如遺傳性血紅蛋白病的病理機(jī)制探索、造血干細(xì)胞分化過(guò)程中血紅蛋白轉(zhuǎn)換的分子研究，以及藥物開發(fā)中對(duì)胎兒血紅蛋白誘導(dǎo)療法的效果評(píng)估，為血液學(xué)基礎(chǔ)研究和疾病模型構(gòu)建提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

HBF
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, lysate for RBC
檢測(cè)范圍：

125 ng/mL-2000 ng/mL
靈敏度：

62.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HBF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	91-99
1:40	Average %	103
	Range %	99-107
1:80	Average %	98
	Range %	93-103
1:160	Average %	92
	Range %	87-97

回收率：

The recovery of human HBF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	89	84-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.091	0.098	0.095
1000	0.175	0.171	0.173
500	0.301	0.305	0.303
250	0.599	0.596	0.598
125	1.265	1.211	1.238
0	2.244	2.201	2.223

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.