Human enkephalin,ENK ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人腦啡肽(ENK)酶聯(lián)免疫試劑盒（CSB-E09117h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的ENK含量。人腦啡肽（ENK）是一種內源性阿片肽，具有鎮(zhèn)痛、調節(jié)情緒和行為等功能。其研究機制涉及與腦內阿片受體結合，通過抑制疼痛信號傳遞和調節(jié)神經遞質來發(fā)揮作用。靶點背景研究顯示，ENK主要作用于μ、δ、κ和痛敏肽受體，其生物學功能廣泛，與多種生理和病理過程相關。試劑盒檢測范圍為0.05 ng/mL-10 ng/mL，適用于科研領域對神經遞質調控機制的探索；可應用于神經生物學、藥理學研究中ENK動態(tài)變化的檢測，或用于評估藥物干預、行為學模型對腦啡肽系統(tǒng)的影響，為探索神經系統(tǒng)功能提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

ENK
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.05 ng/mL-10 ng/mL
靈敏度：

0.025 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-99
1:2	Average %	93
	Range %	87-100
1:4	Average %	93
	Range %	85-99
1:8	Average %	90
	Range %	82-102

回收率：

The recovery of human ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	93-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.