Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人細胞角蛋白21-1片段(CYFRA21-1)酶聯免疫試劑盒（CSB-E09606h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CYFRA21-1含量。CYFRA21-1是一種細胞角蛋白片段，廣泛分布于正常組織中。當細胞癌變時，角蛋白被降解并釋放到血液中，CYFRA21-1水平升高。它作為肺癌，尤其是鱗狀細胞癌的標志物，可用于診斷和預后評估。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，本產品適用于基礎醫學研究中腫瘤標志物表達水平檢測、疾病機制探索或藥物開發過程中的生物標志物動態監測，為體外實驗提供血清/血漿樣本中CYFRA21 - 1濃度的可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CYFRA21-1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	98
	Range %	91-105
1:4	Average %	99
	Range %	94-110
1:8	Average %	92
	Range %	86-99

回收率：

The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	80-98
EDTA plasma (n=4)	97	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.