Human apelin 36(AP36)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人apelin 36(AP36)酶聯(lián)免疫試劑盒（CSB-E13566h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的AP36含量。AP36是一種在多囊卵巢綜合征（PCOS）患者中發(fā)現(xiàn)的肽，其水平與BMI呈正相關(guān)，可能與PCOS的發(fā)病機(jī)制無直接關(guān)系，但可能作為受BMI影響的脂肪因子參與其中。研究顯示，肥胖PCOS患者的AP36水平高于非肥胖PCOS患者，提示其在肥胖相關(guān)PCOS中的作用。試劑盒檢測范圍為22.86 pg/mL-400 pg/mL，該產(chǎn)品可穩(wěn)定檢測復(fù)雜生物樣本中的低豐度AP36，為心血管疾病機(jī)制研究、代謝調(diào)控通路分析、肥胖或糖尿病模型構(gòu)建等科研項(xiàng)目提供可靠工具，尤其適用于動(dòng)態(tài)監(jiān)測疾病模型中AP36的分泌變化或藥物干預(yù)后的生物標(biāo)志物響應(yīng)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AP36
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

22.86 pg/mL-400 pg/mL
靈敏度：

14.29 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AP36 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	89-106
1:2	Average %	95
	Range %	92-99
1:4	Average %	88
	Range %	85-92
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of human AP36 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	93	91-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.