Human antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit
產品詳情
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產品描述：
人抗利尿激素/血管加壓素/精氨酸加壓素(ADH/VP/AVP)酶聯(lián)免疫試劑盒（CSB-E09080h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AVP含量。AVP即精氨酸加壓素，在人體生理中起重要作用。它參與調節(jié)水鹽平衡、血管收縮等。研究上，聚焦其在相關受體的作用機制，探索其與疾病如尿崩癥、心血管病等關聯(lián)，旨在開發(fā)針對AVP靶點的藥物以治療相關病癥。試劑盒檢測范圍為1.25 pg/mL-80 pg/mL，科研人員可將其應用于內分泌調控研究、心血管疾病動物模型評估或水鹽代謝相關實驗，特別適用于探究下丘腦 - 垂體軸功能、滲透壓調節(jié)機制以及病理狀態(tài)下體液失衡的分子基礎，為神經內分泌領域的體外實驗研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
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別名：
AVP ELISA Kit; ARVP ELISA Kit; VPVasopressin-neurophysin 2-copeptin ELISA Kit; AVP-NPII) [Cleaved into: Arg-vasopressin ELISA Kit; Arginine-vasopressin); Neurophysin 2 ELISA Kit; Neurophysin-II); Copeptin] ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
1.25 pg/mL-80 pg/mL
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靈敏度：
0.312 pg/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Neuroscience
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 83 | |
| Range % | 81-99 | |
| 1:2 | Average % | 95 | |
| Range % | 90-105 | |
| 1:4 | Average % | 85 | |
| Range % | 80-91 | |
| 1:8 | Average % | 92 | |
| Range % | 80-98 | |
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回收率：
| The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 85-98 | |
| EDTA plasma (n=4) | 94 | 90-105 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | pg/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.567 | 2.502 | 2.535 | 2.393 | | | 40 | 1.851 | 1.816 | 1.834 | 1.692 | | | 20 | 1.301 | 1.243 | 1.272 | 1.130 | | | 10 | 0.852 | 0.809 | 0.831 | 0.689 | | | 5 | 0.532 | 0.514 | 0.523 | 0.381 | | | 2.5 | 0.383 | 0.376 | 0.380 | 0.238 | | | 1.25 | 0.247 | 0.239 | 0.243 | 0.101 | | | 0 | 0.145 | 0.139 | 0.142 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AVP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled AVP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據處理：
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貨期：
3-5 working days
靶點詳情
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功能：
Specifically binds vasopressin.; Has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2).
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基因功能參考文獻：
- Serum copeptin concentrations did not vary between the pregnancies complicated by intrahepatic cholestasis of pregnancy and the healthy pregnancy control group. PMID: 28552021
- Copeptin appears to be an independent predictor of long-term mortality in a selected population of patients suspected for an acute coronary syndrome (ACS). In addition, copeptin may be considered as an early marker for the identification of patients at higher risk of the development of heart failure at long term in patients with acute chest pain. PMID: 29619130
- Increased levels of NT-proBNP seems to be a better biomarker of multidrug antihypertensive therapy requirement than CT-proAVP. PMID: 29190613
- High AVP expression is associated with gestational diabetes insipidus. PMID: 29378555
- Plasma concentration of copetin demonstrated associations with stroke occurrence in a West African cohort but was not associated with stroke severity or mortality. PMID: 29074065
- Data suggest that corticotrophin-releasing hormone (CRH) is able to stimulate copeptin release in healthy controls suggesting direct interaction of CRH/CRH-receptor signaling and vasopressin; these interactions appear to be altered in patients with pituitary disease; copeptin may be serum biomarker of altered CRH/CRH-receptor signaling in pituitary diseases. PMID: 28795329
- copeptin might have a potential role in the pathogenesis of restless legs syndrome and be a biomarker for this disease. PMID: 29148047
- Serum copeptin is a strong prognostic marker in both chronic obstructive pulmonary disease and acute heart failure. PMID: 29100503
- Hypothalamic suprachiasmatic nucleus -AVP-ir showed a significant negative correlation with age in the control group and in the male, but not in the female depression group. PMID: 28608287
- elevated plasma copeptin level is associated with an increased risk of heart failure (HF) and all-cause mortality in patients with HF. PMID: 28244638
- Resistin, but not copeptin levels are higher in acute ischemic stroke patients early after the stroke onset, than in age and gender matched stroke-free controls. Moreover, higher copeptin concentrations are predictive of poor short term functional outcome after ischemic stroke PMID: 28845746
- Cord blood copeptin concentration does not seem to be a promising marker of acute kidney injury in asphyxiated neonates. PMID: 27590891
- Elevated cord blood copeptin has high potential to become a routinely used biomarker for acute birth asphyxia and neonatal distress. PMID: 28351056
- Copeptin seems to be a promising independent biomarker for predicting the functional outcome and all-cause mortality within 3 months or 1 year after acute ischemic stroke--{REVIEW} PMID: 27904159
- Data suggest that men with type 1 diabetes and albuminuria had greater serum copeptin concentrations than men with normo-albuminuria. PMID: 27979439
- associated with significantly greater annual decline of glomerular filtration rate PMID: 27347674
- Copeptin levels are elevated in cerebral infarction, intracranial hemorrhage and subarachnoid hemorrhage but cannot be used in their differential diagnosis. PMID: 28164562
- High circulating copeptin and decline in glomerular filtration rate indicate greater risk of new onset chronic kidney disease. (Review) PMID: 28714397
- MR-proADM but not copeptin was significantly associated with prognosis of COPD exacerbations at 30 days. PMID: 28408815
- vasopressin and the glycopeptide - mediate physiological aggregation of the wild-type hormone precursor into secretory granules and the pathological fibrillar aggregation of disease mutants in the endoplasmic reticulum. PMID: 28122547
- The inability to clear misfolded proAVP with highly reactive cysteine thiols in the absence of Sel1L-Hrd1 ERAD causes proAVP to accumulate and participate in inappropriate intermolecular disulfide-bonded aggregates, promoted by the enzymatic activity of protein disulfide isomerase (PDI). PMID: 28920920
- High copeptin expression is associated with hypoxic-ischemic encephalopathy. PMID: 28931055
- Copeptin and osmolality were unaffected by sitagliptin treatment in type 2 diabetics with acute coronary syndrome. PMID: 27190088
- Copeptin levels are highest among acute myocardial infarction patients with glucose disturbances and predict an adverse prognosis in unadjusted analyses. PMID: 28118730
- high serum copeptin level is associated with disease severity and progression in IgA nephropathy. PMID: 28057871
- Copeptin was independently associated with an increased risk of incident stroke and CVD mortality in men with diabetes, but not in men without diabetes. PMID: 27312697
- Copeptin level at admission predicts final infarct size in STEMI patients. PMID: 27344134
- Copeptin (>/=9.6 pmol/L) was associated with significantly higher rates of myocardial injury and improved risk stratification in patients scheduled for noncardiac surgery with nonelevated preoperative troponin. PMID: 27870734
- It seems reasonable to indicate genetic testing of patients with isolated neurohypophyseal diabetes insipidus occurring during childhood or adolescence without a family history and without any identifiable cause as mutations of the AVP gene have been previously detected in children, who were thought to have idiopathic disease PMID: 27539621
- High plasma copeptin was associated with reduced insulin sensitivity and an increased risk for IFG/T2DM diabetes in this community-based cohort. Moreover, in men, allelic associations support a causal role for vasopressin in these disorders. PMID: 27049477
- Copeptin was elevated in, and independently predicted prognosis in Heart Failure PMID: 27523461
- Baseline measurements of Copeptin in patients undergoing renal sympathetic denervation (RDN) for resistant hypertension have no predictive value for response to RDN. PMID: 27775435
- Copeptin/UNa ratio may be used as a potential biomarker of Syndrome of Inappropriate Antidiuretic Hormone (SIADH in patients with tick-borne encephalitis (TBE) . Copeptin concentration is significantly higher in patients with TBE than in viral meningitis of other origin, especially in patients aged 18-34 and >49 years old. Copeptin does not differentiate TBE of mild and severe course. PMID: 27882774
- Copeptin and troponin T measurement could potentially improve the prehospital diagnostic and prognostic classification of patients with a suspected AMI. PMID: 27903076
- The early polyuria in recessive central diabetes insipidus contrasts with the delayed presentation in patients with monoallelic AVP mutations. PMID: 26565711
- Heart Failure is a complex syndrome with the differential integration of stimulatory and inhibitory inputs to the AVP/copeptin secretory system. PMID: 27396431
- copeptin may be a good biomarker for metabolic syndrome. PMID: 27928437
- The present study reports the genetic, clinical and biochemical characteristics of patients with Familial neurohypophyseal diabetes insipidus caused by five novel mutations in AVP. PMID: 27513365
- The combination of a clinical model with copeptin and NTproBNP, which are available in the Emergency Department, is able to prognose early mortality in patients with an episode of Acute Heart Failure. PMID: 28069402
- High Serum copeptin levels are associated with respiratory infections. PMID: 27171391
- A high serum copeptin concentration predicts transplant-free survival, particularly at 6 months, independently of liver-specific scoring systems in a heterogeneous population of hospitalized cirrhotic patients. PMID: 26502363
- A direct release of copeptin is not detectable from the human heart in acute myocardial infarction. PMID: 26864512
- plasma copeptin levels can be regarded for promising marker of severity of acute pulmonary embolism and show potential in risk stratification of these patients PMID: 26438275
- AVP gene mutation is associated with neurohypophyseal diabetes insipidus. PMID: 26208472
- Serum copeptin was found to be increasing significantly in cases of myocardial ischemia detected by myocardial perfusion scintigraphy. PMID: 26100831
- Serum copeptin is elevated in patients with acute pulmonary embolism. PMID: 26711465
- There was no significant difference in serum copeptin between patients with vasovagal syncope, epilepsy, and controls. PMID: 26641207
- Data from a population in Sweden suggest association between elevated copeptin plasma levels at time of diagnosis of type 2 diabetes and later development (within 10-12 years) of renal insufficiency and progression to chronic kidney disease stage 3. PMID: 26321369
- This study evaluated the relationships among copeptin, ischemia-modified albumin (IMA), and extent of myocardial injury in patients with acute carbon monoxide poisoning. PMID: 26345979
- Low serum sodium was associated with an increased risk of cardiovascular and all-cause mortality in type 2 diabetes, but association was not explained by copeptin. PMID: 26201002
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相關疾病：
Diabetes insipidus, neurohypophyseal (NDI)
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亞細胞定位：
Secreted.
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蛋白家族：
Vasopressin/oxytocin family
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數(shù)據庫鏈接：
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