Human alpha-L-fucosidase,AFU ELISA Kit

Human alpha-L-fucosidase,AFU ELISA Kit

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中文名稱：

人αL巖藻糖苷酶(AFU)酶聯免疫試劑盒
貨號：

CSB-E08348h
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人αL巖藻糖苷酶(AFU)酶聯免疫試劑盒（CSB-E08348h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FUCA2含量。FUCA2 是一個靶點。其相關它在機體特定生理過程中可能發(fā)揮重要作用。研究機制上，科學家可能會從基因表達調控、蛋白功能等角度展開研究，探究其在疾病發(fā)生發(fā)展中的作用，以尋找潛在治療靶點和干預措施。試劑盒檢測范圍為31.25 μU/mL-2000 μU/mL，適用于體外科研實驗中AFU的精準定量分析；可為肝臟病理機制研究、腫瘤標志物篩選、代謝性疾病模型構建等基礎科研提供可靠數據支持；適用于實驗室批量檢測需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Alpha L fucosidase 2 ELISA Kit; Alpha L fucosidase fucohydrolase 2 ELISA Kit; Alpha-L-fucosidase 2 ELISA Kit; Alpha-L-fucoside fucohydrolase 2 ELISA Kit; dJ20N2.5 ELISA Kit; FUCA 2 ELISA Kit; FUCA2 ELISA Kit; FUCO2 ELISA Kit; FUCO2_HUMAN ELISA Kit; Fucosidase alpha L 2 plasma ELISA Kit; Fucosidase; alpha-L; plasma ELISA Kit; MGC1314 ELISA Kit; Plasma alpha L fucosidase ELISA Kit; Plasma alpha-L-fucosidase ELISA Kit; RP1 20N2.5 ELISA Kit
縮寫：

FUCA2
Uniprot No.：

Q9BTY2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 μU/mL-2000 μU/mL
靈敏度：

7.8 μU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AFU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	88
	Range %	81-95
1:40	Average %	91
	Range %	86-97
1:80	Average %	97
	Range %	91-102
1:160	Average %	102
	Range %	97-107

回收率：

The recovery of human AFU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-110
EDTA plasma (n=4)	93	85-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µU/ml	OD1	OD2	Average	Corrected
2000	2.720	2.546	2.633	2.546
1000	2.235	2.200	2.218	2.131
500	1.554	1.633	1.594	1.507
250	0.821	0.845	0.833	0.746
125	0.471	0.445	0.458	0.371
62.5	0.311	0.302	0.307	0.220
31.25	0.185	0.188	0.187	0.100
0	0.084	0.090	0.087

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Evaluating new biomarkers for diabetic nephropathy: Role of α2-macroglobulin, podocalyxin, α-L-fucosidase, retinol-binding protein-4, and cystatin C JJ Li,World journal of diabetes,2024

功能：

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
基因功能參考文獻：
1. Genetic polymorphisms within the FUCA2 and IL18 gene regions are also associated with diastolic function in SCD, likely by affecting expression levels of the genes PMID: 27636371
2. serum alpha-L-fucosidase was identified as a useful marker for close monitoring of patients during post-treatment follow-up PMID: 18521898
3. Findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for diagnosis and therapy of H. pylori-related diseases. PMID: 19666478
亞細胞定位：

Secreted.
蛋白家族：

Glycosyl hydrolase 29 family
數據庫鏈接：

HGNC: 4008

OMIM: 136820

KEGG: hsa:2519

STRING: 9606.ENSP00000002165

UniGene: Hs.17680

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.