Human Tumor necrosis factor-inducible gene 6 protein(TNFAIP6) ELISA kit
產品詳情
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產品描述：
人腫瘤壞死因子誘導基因6蛋白(TNFAIP6)酶聯免疫試劑盒（CSB-EL023959HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNFAIP6含量。TNFAIP6 靶點具有重要生物學意義。它可能參與炎癥、組織修復等生理過程。相關研究聚焦于其在疾病發生發展中的作用機制，探索其作為治療靶點的潛力，如在炎癥性疾病等領域，為開發針對性藥物提供理論基礎。試劑盒檢測范圍為47 ng/ml- 3000 ng/ml，適用于基礎科研中探索TNFAIP6在炎癥性疾病、纖維化病變或腫瘤發展中的表達規律，亦可用于評估體外實驗模型中藥物干預或基因調控對該靶點的影響。為研究TNFAIP6在病理生理過程中的動態變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
Hyaluronate binding protein ELISA Kit; Hyaluronate-binding protein ELISA Kit; TNF alpha induced protein 6 ELISA Kit; TNF alpha-induced protein 6 ELISA Kit; TNF stimulated gene 6 protein ELISA Kit; TNF-stimulated gene 6 protein ELISA Kit; TNFAIP 6 ELISA Kit; Tnfaip6 ELISA Kit; TSG 6 ELISA Kit; TSG-6 ELISA Kit; TSG6_HUMAN ELISA Kit; Tumor necrosis factor alpha induced protein 6 ELISA Kit; Tumor necrosis factor alpha inducible protein 6 ELISA Kit; Tumor necrosis factor alpha-induced protein 6 ELISA Kit; Tumor necrosis factor inducible gene 6 protein ELISA Kit; Tumor necrosis factor stimulated gene 6 protein ELISA Kit; Tumor necrosis factor-inducible gene 6 protein ELISA Kit
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縮寫：
TNFAIP6
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
47 ng/ml - 3000 ng/ml
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靈敏度：
11.75 ng/ml
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Cancer
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) | |
1:1 | Average % | 99 | |
Range % | 92-106 | |
1:2 | Average % | 85 | |
Range % | 81-92 | |
1:4 | Average % | 89 | |
Range % | 84-94 | |
1:8 | Average % | 95 | |
Range % | 88-101 | |
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回收率：
The recovery of human TNFAIP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 94 | 89-100 | |
EDTA plasma (n=4) | 88 | 81-95 | |
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標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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ng/ml | OD1 | OD2 | Average | Corrected | |
3000 | 2.715 | 2.634 | 2.674 | 2.546 | |
1500 | 2.032 | 2.167 | 2.099 | 1.972 | |
750 | 1.329 | 1.278 | 1.303 | 1.175 | |
375 | 0.786 | 0.749 | 0.767 | 0.640 | |
187.5 | 0.427 | 0.411 | 0.419 | 0.291 | |
94 | 0.294 | 0.278 | 0.286 | 0.158 | |
47 | 0.227 | 0.217 | 0.222 | 0.094 | |
0 | 0.131 | 0.125 | 0.128 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TNFAIP6 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TNFAIP6 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
產品評價
樣品類型：血漿（抗凝劑） EDTA
樣品信息：人
稀釋比：沒有稀釋
產品評價： ELISA結果正常組和患病組有明顯差異
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