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中文名稱：人轉膠蛋白2(TAGLN2)酶聯免疫試劑盒
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貨號：CSB-EL023106HU
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人轉膠蛋白2(TAGLN2)酶聯免疫試劑盒（CSB-EL023106HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細胞裂解物樣本中的TAGLN2含量。TAGLN2是一種與肌動蛋白結合的蛋白，被認為具有腫瘤抑制功能。其研究機制涉及調節T細胞脂質代謝，通過促進脂肪酸結合蛋白5（FABP5）的運輸至T細胞表面，增強T細胞在腫瘤微環境中的抗腫瘤功能。TAGLN2的表達受腫瘤微環境中的內質網應激反應調節，其過表達可能有助于提高癌癥免疫療法的療效。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研人員可利用開展疾病相關分子機制研究，例如通過檢測不同處理條件下細胞裂解液或體液樣本中TAGLN2的動態變化，探索其在細胞運動、炎癥反應或代謝調控中的功能，也可用于藥物開發過程中靶點蛋白表達水平的定量評估。本產品適用于生命科學基礎研究與轉化醫學領域的蛋白定量需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：CDABP0035 ELISA Kit; epididymis tissue protein Li 7e ELISA Kit; HA1756 ELISA Kit; KIAA0120 ELISA Kit; SM22-alpha homolog ELISA Kit; TAGL2_HUMAN ELISA Kit; Tagln2 ELISA Kit; Transgelin-2 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, cell lysates
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檢測范圍：0.312 ng/mL-20 ng/mL
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靈敏度：0.078 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 87-92 1:2 Average % 101 Range % 98-103 1:4 Average % 92 Range % 85-96 1:8 Average % 99 Range % 93-102 -
回收率：
The recovery of human TAGLN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 92-98 EDTA plasma (n=4) 96 92-99 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 2.398 2.367 2.383 2.228 10 1.672 1.576 1.624 1.469 5 1.071 1.062 1.067 0.912 2.5 0.624 0.618 0.621 0.466 1.25 0.427 0.411 0.419 0.264 0.625 0.319 0.306 0.313 0.158 0.312 0.251 0.240 0.246 0.091 0 0.156 0.154 0.155 -
數據處理：
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貨期：3-5 working days
引用文獻
- Identification of metabolic biomarkers in atrial fibrillation patients via the integrated application of proteomics and metabolomics X Chao,/,2024
- Identification and verification of transgelin-2 as a potential biomarker of tumor-derived lung-cancer endothelial cells by comparative proteomics Jin H. et al,J Proteomics,2015
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