Human Tenascin-N(TNN) ELISA kit

Human Tenascin-N(TNN) ELISA kit

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中文名稱：

人肌腱蛋白N(TNN)酶聯免疫試劑盒
貨號：

CSB-EL024008HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人肌腱蛋白N(TNN)酶聯免疫試劑盒（CSB-EL024008HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TNN含量。人肌腱蛋白N（TNN）是一種重要的多肽糖蛋白，在生物體內發揮著多種生物學功能，如組織或細胞黏附、調控細胞遷移和生存等。其研究機制涉及基因表達、結構和功能等多方面，對理解其生物學功能和疾病發生具有重要意義。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外培養細胞功能研究、動物模型組織分析以及生物體液樣本中TNN的動態監測，為探索其在炎癥反應、纖維化疾病或腫瘤發生發展中的分子機制提供可靠工具，支持科研工作者開展基礎醫學研究中的蛋白表達定量分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TNN ELISA Kit; TNW ELISA Kit; Tenascin-N ELISA Kit; TN-N ELISA Kit; Tenascin-W ELISA Kit; TN-W ELISA Kit
縮寫：

TNN
Uniprot No.：

Q9UQP3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-99
1:2	Average %	102
	Range %	97-105
1:4	Average %	94
	Range %	88-98
1:8	Average %	98
	Range %	93-102

回收率：

The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	92	86-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.022	2.001	2.012	1.855
5	1.733	1.812	1.773	1.616
2.5	1.307	1.288	1.298	1.141
1.25	0.989	0.973	0.981	0.824
0.625	0.673	0.666	0.670	0.513
0.312	0.466	0.454	0.460	0.303
0.156	0.301	0.312	0.307	0.150
0	0.156	0.158	0.157

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1. Involved in neurite outgrowth and cell migration in hippocampal explants. During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells. Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells.
基因功能參考文獻：
1. This study demonstrated that the genetic defect was totally or partly clarified in 21 patients with nine of them having potential disease-causing mutations in TTN. PMID: 26627873
2. Results show that tenascin-W acts as a niche component for breast cancer metastasis to bone by supporting cell migration and cell proliferation of the cancer cells. PMID: 25868708
3. The expression of tenascin-W is dependent on p38MAPK and JNK signaling pathways in mammary tumors. (tenascin-W) PMID: 15592496
4. data imply that tenascin-W expression in the activated tumor stroma facilitates tumorigenesis by supporting the migratory behavior of breast cancer cells PMID: 17909022
5. results reveal a clear association between elevated levels of tenascin-W and the presence of colorectal cancer and breast cancer PMID: 18306355
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Tenascin family
組織特異性：

Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested. In brain tumors, detected around the endothelial cell layer of the clood vessel
數據庫鏈接：

HGNC: 22942

OMIM: 617472

KEGG: hsa:63923

STRING: 9606.ENSP00000239462

UniGene: Hs.156369

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.