Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
86
Range %
81-90
1:2
Average %
98
Range %
93-101
1:4
Average %
95
Range %
90-100
1:8
Average %
94
Range %
86-97
回收率:
The recovery of human SDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
90-97
EDTA plasma (n=4)
90
87-94
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Methylation of SFRP1, SFRP2, SDC2, and PRIMA1 promoter sequences was observed in 85.1%, 72.3%, 89.4%, and 80.9% of plasma samples from patients with Colorectal cancer and 89.2%, 83.8%, 81.1% and 70.3% from adenoma patients, respectively. PMID: 28753106
Emerging roles of SDC2 in epithelial and mesenchymal cancer progression has been summarized. (Review) PMID: 28940845
data suggest that syndecan-2 induces extracellular shedding of E-cadherin and supports the acquisition of a fibroblast-like morphology by regulating MMP-7 expression in a colon cancer cell line PMID: 27270030
SDC2 is overexpressed in Leri's pleonosteosis. The minor allele of a missense SDC2 variant, p.Ser71Thr, could confer protection against systemic sclerosis. PMID: 24442880
In this review we considered the function of syndecan-2, the structure of syndecan-2 and its role in the formation of amyloid plaques. PMID: 25916113
Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID: 24804215
HIV matrix protein p17 is pro-fibrogenic through its interactions both with CXCR2 and syndecan-2 on activated HSCs PMID: 24736615
The results demonstrate the importance of PDZ-binding domain of syndecan-2 for controlling LFA-1 affinity and cell adhesion. PMID: 24662262
SDC2 expression was higher in skin melnaoma cells. It enhanced tyrosinase activity by increasing the membrane and melanosome localization of protein kinase CbetaII. UVB-induced up-regulation was required for UVB-induced melanin synthesis. PMID: 24472179
syndecan 2 shows no changes associated to histological grade of Prostate cancer PMID: 24424718
RhoA, but not RhoC was shown to be essential for the anchored phenotype of breast carcinoma cells that accompanied siRNA-mediated loss of syndecan-2. PMID: 24447566
RKIP, LOX and SDC2 are coordinately regulated and collectively encompass a prognostic signature for metastasis-free survival in ER-negative breast cancer patients PMID: 23975428
Taken together, these data suggest that IL-1alpha regulates extracellular domain shedding of syndecan-2 through regulation of the MAP kinase-mediated MMP-7 expression in colon cancer cells. PMID: 24613844
Clinical validation of SDC2 methylation in serum DNA by quantitative methylation-specific PCR demonstrated a high sensitivity of 87.0% (95% CI, 80.0% to 92.3%) in detecting cancers. PMID: 23747112
DNA from 4 HPV positive and 4 HPV negative fresh frozen primary HNSCC were subject to comprehensive genome-wide methylation profiling.Pathway analysis of 1168 methylated genes showed 8 signal transduction pathways (SDC2) of which 62% are hypermethylated. PMID: 23736812
Taken together, these results suggest that the extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin. PMID: 23333331
SDC-2 modulates TGFbeta2 transcriptional regulation via Smad2 signaling to facilitate fibrosarcoma cell adhesion. PMID: 23297089
The repression of syndecan-2 by Wnt/beta-catenin/TCF signaling contributes to the resistance of osteosarcoma cells to doxorubicin. PMID: 22550000
lower TCR/CD3 surface levels caused by syndecan-2 led to reduced TCR/CD3 responsiveness PMID: 22881146
MC1R regulates melanoma cell migration via inhibition of syndecan-2 expression. PMID: 22493442
SDC-2 is a novel (perineural) invasion-associated gene in PDAC which cooperates with K-ras to induce a more invasive phenotype. PMID: 22471946
Notch receptors control their own activity by inducing the expression of syndecan-2, which then acts to propagate Notch signaling by direct receptor interaction. PMID: 22437834
a specific glycochain is a receptor for dengue virus strain DEN2 16681 PMID: 22170634
the data suggest that MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells. PMID: 22227189
Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to syndecan-2 extracellular domain, with downstream beta1 integrin-mediated adhesion and cytoskeletal organization. PMID: 21813734
syndecan-2 was expressed preferentially in basal cells in benign tissue. In prostate cancer samples, syndecan 2 was expressed in granular-cytoplasmic localisation. PMID: 21317913
HIV-1 p17 matrix protein interacts with heparan sulfate side chain of CD44v3, syndecan-2, and syndecan-4 proteoglycans expressed on human activated CD4+ T cells affecting tumor necrosis factor alpha and interleukin 2 production. PMID: 21482826
the cytoplasmic domain of syndecan-2 regulates colon cancer cell migration via interaction with syntenin-1. PMID: 21569759
The findings demonstrate that LRP1 and HSPG function in a cooperative manner to mediate cellular Abeta uptake and define a major pathway through which Abeta gains entry to neuronal cells. PMID: 21289173
syndecan-2 and syndecan-4 regulate the compensatory effect of TG-FN on osteoblast cell adhesion and actin cytoskeletal formation in the presence of RGD peptides. PMID: 21036168
RGD-independent cell adhesion via a tissue transglutaminase 2-fibronectin matrix promotes fibronectin fibril deposition and requires syndecan-4/2 and {alpha}5{beta}1 integrin co-signaling. PMID: 20929862
Results support a potential role for syndecan-2 in pancreatic carcinogenesis and cancer progression. Expression of syndecan-2 might serve as a prognostic marker. PMID: 20683009
Study results supported the potential role of SDC2 in prostatic carcinogenesis and cancer progression. PMID: 19786981
these results suggest that syndecan-2 regulates cell migration of colon carcinoma cells through Tiam1-dependent Rac activation in colon cancer cells. PMID: 19962968
syndecan's interactions with both CASK and neurofibromin are dependent on syndecan homodimerization. PMID: 20006588
Identification of integrin alpha(M)beta(2) as an adhesion receptor on human peripheral blood monocytes for Cyr61 and connective tissue growth factor: immediate-early gene products expressed in atherosclerotic lesions.(integrin alpha(M)beta(2)) PMID: 12036876
role in mediating adhesion and proliferation of colon carcinoma cells PMID: 12055189
Ezrin N-terminal domain binds to the syndecan-2 cytoplasmic domain with an estimated K(D) of 0.71 microM and without the requirement of other proteins PMID: 12860416
Mechanisms for the regulation of HSPG have been studied. PMID: 12860968
Interleukin-8 binds to syndecan-2 on vascular endothelial cells. PMID: 14527339
the HSPG T allele is a risk factor for the development of severe diabetic nephropathy in type 2 diabetic patients PMID: 14674716
Syndecan-2 induces osteoblastic cell apoptosis through the JNK/Bax apoptotic pathway and the cytoplasmic domain of syndecan-2 is required for this action. PMID: 15936998
the transmembrane domains are sufficient for inducing dimerization and transmembrane domain-induced oligomerization is crucial for syndecan-2 and syndecan-4 functions PMID: 16253987
molecular complex most likely to obstruct RACK1 for functional attachment at syndecan-2, as revealed in cells transfected with oncogenic ras PMID: 16997272
HSPGs are expressed on the membrane of myeloid leukemic cell lineages. PMID: 17035092
heparanase-1 expression and heparan sulphate proteoglycan degradation are induced by estradiol in human endometrium PMID: 17261577
these data suggested that both HSPG and CD81 are important for HCV entry. PMID: 17457918
HSPG modulation of BMP signaling in myositis ossificans cells is reported. PMID: 17516498
syndecan-2 acts as a suppressor for MMP-2 activation, causing suppression of metastasis PMID: 17623663
There are pronounced tubulointerstitial HSPG alterations in primary kidney disease, which may affect the inflammatory response. PMID: 18032547