Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

人絲氨酸/蘇氨酸蛋白激酶LMTK3(LMTK3)酶聯(lián)免疫試劑盒（CSB-EL013017HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LMTK3含量。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于基礎(chǔ)科研領(lǐng)域中對疾病機(jī)制探索、藥物靶點驗證或細(xì)胞信號通路研究，例如通過檢測不同處理條件下細(xì)胞或動物模型樣本中LMTK3的動態(tài)變化，分析其在腫瘤進(jìn)展中的功能，或評估潛在治療藥物對該靶點的調(diào)控效應(yīng)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LMTK3 ELISA Kit; KIAA1883 ELISA Kit; TYKLM3 ELISA Kit; Serine/threonine-protein kinase LMTK3 ELISA Kit; EC 2.7.11.1 ELISA Kit; Lemur tyrosine kinase 3 ELISA Kit
縮寫：

LMTK3
Uniprot No.：

Q96Q04
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.7 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LMTK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of human LMTK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	105	100-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription. Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons.
亞細(xì)胞定位：

Membrane; Single-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Golgi apparatus membrane. Note=Punctate pattern in cell projections.
蛋白家族：

Protein kinase superfamily, Tyr protein kinase family
數(shù)據(jù)庫鏈接：

HGNC: 19295

UniGene: Hs.207426