Human Protein Wnt-7b(WNT7B) ELISA kit

Human Protein Wnt-7b(WNT7B) ELISA kit

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中文名稱：

人Wnt-7b蛋白(WNT7B)酶聯免疫試劑盒
貨號：

CSB-EL026142HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人Wnt-7b蛋白(WNT7B)酶聯免疫試劑盒（CSB-EL026142HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿樣本中的WNT7B含量。WNT7B 是重要靶點。它參與 Wnt 信號通路，在胚胎發育、細胞增殖與分化等方面發揮關鍵作用。研究其機制有助于揭示疾病發生發展原理，為相關疾病如癌癥等的診斷、治療及藥物研發提供新方向與潛在靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于基礎研究領域，如探究Wnt信號通路在組織修復、干細胞分化或疾病模型中的作用機制，尤其適合需要從復雜生物樣本（如炎癥相關組織勻漿或循環系統樣本）中定量分析WNT7B表達變化的實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Protein Wnt-7b ELISA Kit; Wingless related MMTV integration site 7B ELISA Kit; Wingless type MMTV integration site family member 7B ELISA Kit; WNT ELISA Kit; WNT7B ELISA Kit; WNT7B_HUMAN ELISA Kit
縮寫：

WNT7B
Uniprot No.：

P56706
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma,tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Stem Cells
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human WNT7B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	102
	Range %	95-108
1:2	Average %	96
	Range %	91-100
1:4	Average %	87
	Range %	82-93
1:8	Average %	95
	Range %	88-102

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.532

2.634

2.583

2.444

1.683

1.767

1.725

1.586

2.5

0.901

0.928

0.915

0.775

1.25

0.554

0.579

0.567

0.427

0.625

0.363

0.349

0.356

0.217

0.312

0.272

0.259

0.265

0.126

0.156

0.189

0.194

0.191

0.052

0.143

0.136

0.139

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.
基因功能參考文獻：
1. We identified a novel genome-wide significant association rs10453441 in the WNT7B gene on chromosome 22 as a novel SNP for central corneal thickness in Latinos. PMID: 28171582
2. the present study detected abnormal upregulation in the levels of Wnt2b and Wnt7b, and hypothesized that the alterations may be due to the ectopic opening of chromatin structure. PMID: 26548512
3. WNT7B as a novel susceptibility gene for axial length and corneal curvature and is associated with myopia and extreme myopia. PMID: 25823570
4. Results illustrated the critical role of myeloid WNT7B in tumor progression, acting at the levels of angiogenesis, invasion, and metastasis. PMID: 24638982
5. Results suggest that AR-regulated WNT7B signaling is critical for the growth of CRPC and development of the osteoblastic bone response characteristic of advanced prostate cancer. PMID: 23386686
6. WNT7B can serve as a primary determinant of differential Wnt/beta-catenin activation in pancreatic adenocarcinoma. PMID: 23416978
7. Wnt7B was expressed at high concentrations in regions of active hyperplasia, metaplasia, and fibrotic change in idiopathic pulmonary fibrosis patients. PMID: 22838404
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亞細胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Wnt family
組織特異性：

Moderately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
數據庫鏈接：

HGNC: 12787

OMIM: 601967

KEGG: hsa:7477

STRING: 9606.ENSP00000341032

UniGene: Hs.512714

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.