Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

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中文名稱：

人凝血酶原前體蛋白(PIVKA-II)酶聯免疫試劑盒
貨號：

CSB-E13343h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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引用文獻
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產品描述：

人凝血酶原前體蛋白(PIVKA-II)酶聯免疫試劑盒（CSB-E13343h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腹水、組織勻漿樣本中的PIVKA-II含量。PIVKA-II即異常凝血酶原，是一種腫瘤標志物。在維生素K缺乏或拮抗時產生，在肝癌等腫瘤細胞中高表達。研究機制上，它可反映肝細胞癌的生長和凝血功能異常，為肝癌早期診斷、病情監測和預后評估提供重要參考。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于肝癌發生機制研究、抗腫瘤藥物研發、肝臟功能評估等科研領域，其多樣本兼容性特別適合臨床前研究中不同生物樣本的橫向對比分析，如腫瘤組織勻漿中異常凝血酶原表達檢測，或腹水樣本中的腫瘤標志物水平動態監測，為維生素K代謝研究及肝病相關分子機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PIVKA-II
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, ascitic fluid, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-101
1:2	Average %	97
	Range %	86-101
1:4	Average %	96
	Range %	90-108
1:8	Average %	94
	Range %	82-103

回收率：

The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-104
EDTA plasma (n=4)	97	91-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.467	2.418	2.443	2.327
10	1.962	1.945	1.954	1.838
5	1.342	1.311	1.327	1.211
2.5	0.823	0.772	0.798	0.682
1.25	0.504	0.496	0.500	0.384
0.625	0.331	0.321	0.326	0.210
0.312	0.201	0.189	0.195	0.079
0	0.117	0.114	0.116

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The Usefulness of Vitamin K-Dependent Proteins in the Diagnosis of Colorectal Carcinoma MG Perné,International journal of molecular sciences,2024
Vitamin K Status Based on K1, MK-4, MK-7, and Undercarboxylated Prothrombin Levels in Adolescent and Adult Patients with Cystic Fibrosis: A Cross-Sectional Study P Krzy?anowska,/,2024
Predictive value of protein induced by absence of vitamin K absence or antagonist II, alpha-fetoprotein and gamma-glutamyltransferase/aspartate aminotransferase ratio for the diagnosis of hepatocellular carcinoma in transplantation candidates Amany M. Diab,European Journal Of Gastroenterology & Hepatology,2021
The Diagnostic Value Of AFP, AFP L3 And PIVKA II in Hepatocellular Carcinoma Outcome of Cirrhosis of The Liver HBV And HCV Etiology II PIVKA,European Journal of Molecular & Clinical Medicine,2021
Biological Evidences of Hepatocellular Carcinoma Treatment with 1, 3, 4-oxadiazole-2-thiol as Anticancer Agent Mohamed S Gabry1.et al,Biomolecular Research & Therapeutics,2017
ANNALS EXPRESS: Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis Unic A.et al,Ann Clin Biochem.,2017
Functional deficiency of vitamin K in hemodialysis patients in Upper Silesia in Poland Wyskida K.et al,Int Urol Nephrol.,2016
Diagnostic Performance of Alpha-Fetoprotein, Protein Induced by Vitamin K Absence, Osteopontin, Dickkopf-1 and Its Combinations for Hepatocellular Carcinoma Eun Sun Jang.et al,PLoS One.,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.