Human Protein FAM132A(FAM132A) ELISA kit

Human Protein FAM132A(FAM132A) ELISA kit

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中文名稱：

人蛋白FAM132A(FAM132A)酶聯免疫試劑盒
貨號：

CSB-EL008058HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人蛋白FAM132A(FAM132A)酶聯免疫試劑盒（CSB-EL008058HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的FAM132A含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產品可為研究者分析FAM132A在肥胖、糖尿病相關模型中的表達動態，探索其在細胞能量應激響應機制中的作用提供可靠工具，同時支持腫瘤微環境、神經退行性疾病等領域的蛋白功能研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C1QTNF12 ELISA Kit; C1QDC2 ELISA Kit; CTRP12 ELISA Kit; FAM132AAdipolin ELISA Kit; Adipose-derived insulin-sensitizing factor ELISA Kit; C1q and TNF related protein 12 ELISA Kit; Complement C1q tumor necrosis factor-related protein 12) [Cleaved into: Adipolin fC1QTNF12 ELISA Kit; Adipolin fCTRP12 ELISA Kit; Adipolin full-length form); Adipolin gC1QTNF12 ELISA Kit; Adipolin cleaved form ELISA Kit; Adipolin gCTRP12)] ELISA Kit
縮寫：

FAM132A
Uniprot No.：

Q5T7M4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	85-89
1:2	Average %	99
	Range %	91-102
1:4	Average %	100
	Range %	93-108
1:8	Average %	94
	Range %	90-99

回收率：

The recovery of human FAM132A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.380	2.356	2.368	2.237
1000	1.815	1.776	1.796	1.665
500	1.220	1.198	1.209	1.078
250	0.669	0.666	0.668	0.537
125	0.438	0.424	0.431	0.300
62.5	0.226	0.222	0.224	0.093
31.25	0.181	0.178	0.180	0.049
0	0.134	0.128	0.131

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue.
基因功能參考文獻：
1. Adipolin and adiponectin are cytokines that exert substantial impact on obesity, progression of atherosclerosis, insulin resistance, and glucose metabolism. PMID: 29452073
2. Low serum CTRP12 level is associated with Type 2 diabetes mellitus. PMID: 28207876
3. Coenzyme Q10 reduced HbA1c considerably in overweight and obese patients with diabetes, although interestingly adipolin levels declined simultaneously. In this study, Q10 modulated glucose homeostasis, which was expected to be mediated by increasing adipolin. The similar mechanisms of action of Q10 and adipolin may justify lowering effect of Q10 on adipolin. PMID: 27657997
4. Serum adipolin concentrations were significantly lower in polycystic ovarian syndrome compared with control subjects. PMID: 24612181
5. In subcutaneous adipose tissue explants, insulin significantly increased CTRP12 protein expression and secretion. PMID: 24492466
6. we describe, for the first time, adipolin expression in human chondrocytes as well as the intracellular mechanism involved in its regulation PMID: 22736092
7. Endopeptidase cleavage generates a functionally distinct isoform of C1q/tumor necrosis factor-related protein-12 (CTRP12) with an altered oligomeric state and signaling specificity. PMID: 22942287
8. Reports cloning, expression, regulation and function of mouse adipolin (Fam132a) PMID: 21849507
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亞細胞定位：

[Adipolin fC1QTNF12]: Secreted.; [Adipolin gC1QTNF12]: Secreted.
蛋白家族：

Adipolin/erythroferrone family
組織特異性：

Predominantly expressed by adipose tissues.
數據庫鏈接：

HGNC: 32308

OMIM: 616593

KEGG: hsa:388581

STRING: 9606.ENSP00000329137

UniGene: Hs.197613

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.