Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit
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中文名稱:人前心鈉肽(Pro-ANP)酶聯(lián)免疫試劑盒
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貨號:CSB-E05149h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人前心鈉肽(Pro-ANP)酶聯(lián)免疫試劑盒(CSB-E05149h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Pro-ANP含量。Pro-ANP(前心鈉素原)是一個(gè)具有重要生理意義的靶點(diǎn)。它可反映心臟功能狀態(tài),與心血管系統(tǒng)疾病密切相關(guān)。研究機(jī)制上,主要聚焦于其在調(diào)節(jié)體液平衡、血壓穩(wěn)定等方面的作用,探尋其在疾病發(fā)生發(fā)展中的變化,為相關(guān)疾病治療提供理論依據(jù)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,該產(chǎn)品廣泛應(yīng)用于心血管疾病機(jī)制研究、藥物干預(yù)效果評估、動(dòng)物模型心臟功能分析等科研場景,尤其適用于探索心力衰竭、高血壓及相關(guān)代謝性疾病中Pro - ANP的動(dòng)態(tài)變化規(guī)律,為實(shí)驗(yàn)室提供穩(wěn)定可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:Pro-ANP
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:62.5 pg/mL-4000 pg/mL
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靈敏度:15.6 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 86-97 1:2 Average % 85 Range % 80-89 1:4 Average % 92 Range % 87-98 1:8 Average % 104 Range % 96-108 -
回收率:
The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 84-95 EDTA plasma (n=4) 87 82-93 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 4000 1.943 1.896 1.920 1.766 2000 1.452 1.366 1.409 1.255 1000 0.998 0.968 0.983 0.829 500 0.646 0.657 0.652 0.498 250 0.467 0.454 0.461 0.307 125 0.322 0.306 0.314 0.160 62.5 0.209 0.203 0.206 0.052 0 0.155 0.153 0.154 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Evaluation of Cardiac Troponin I and N‐Terminal pro B – Type Natriuretic Peptide Serum Levels in Patients with Alopecia Areata RESM Marie,Clin Exp Dermatol,2020
- Associations of rs3918242 and rs2285053 MMP-9 and MMP-2 polymorphisms with the risk, severity, and short- and long-term complications of degenerative mitral valve diseases: a 4.8-year prospective cohort study Balistreri C R.et al,Cardiovascular Pathology,2016
相關(guān)問答
■ 常見問題解答
Q:
為檢測Pro-anp水平,所有樣本是否同時(shí)采集并進(jìn)行測試?樣本可以保存多久?
A:
對于CSB-E05149h(Human Pro Atrial Natriuretic Peptide, Pro-ANP ELISA Kit)-96T,我們建議使用新收集的樣本。如果您在這方面有困難,您可以根據(jù)實(shí)驗(yàn)計(jì)劃在適當(dāng)?shù)臈l件下存儲樣本。通常情況下,如果您將在5天內(nèi)使用樣本,建議使用2-8攝氏度保存;如果在1個(gè)月內(nèi)使用,建議使用-20攝氏度保存;長期保存建議使用-80攝氏度,但通常不超過2個(gè)月。期間避免反復(fù)凍融。當(dāng)然您可以根據(jù)自己的實(shí)驗(yàn)需求決定是否需要進(jìn)行分裝。對于第二個(gè)問題,我們沒有進(jìn)行相關(guān)驗(yàn)證,如果條件都允許,建議盡早使用樣本。
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