Human Plexin-C1(PLXNC1) ELISA kit

Human Plexin-C1(PLXNC1) ELISA kit

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中文名稱：

人叢狀蛋白C1(PLXNC1)酶聯免疫試劑盒
貨號：

CSB-EL018225HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人叢狀蛋白C1(PLXNC1)酶聯免疫試劑盒（CSB-EL018225HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PLXNC1含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于探索PLXNC1在神經退行性疾病模型中的功能機制、腫瘤微環境相互作用研究，或作為炎癥相關信號通路研究的工具，為神經科學、免疫學及癌癥生物學領域的體外實驗提供可靠的蛋白定量支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CD232 ELISA Kit; Plexin-C1 ELISA Kit; PLXC1_HUMAN ELISA Kit; Plxnc1 ELISA Kit; Virus-encoded semaphorin protein receptor ELISA Kit
縮寫：

PLXNC1
Uniprot No.：

O60486
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-98
1:2	Average %	93
	Range %	85-97
1:4	Average %	98
	Range %	94-102
1:8	Average %	85
	Range %	80-89

回收率：

The recovery of human PLXNC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	97-108
EDTA plasma (n=4)	87	82-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.016	2.118	2.067	1.873
10	1.735	1.637	1.686	1.492
5	1.164	1.226	1.195	1.001
2.5	0.753	0.775	0.764	0.570
1.25	0.552	0.543	0.548	0.354
0.625	0.413	0.405	0.409	0.215
0.312	0.312	0.304	0.308	0.114
0	0.193	0.195	0.194

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.
基因功能參考文獻：
1. Results indicate that long non-coding RNA (lncRNA) CASC2 can promote PLXNC1 expression by sponging miR-181a, thereby inhibiting the proliferation and invasion of melanoma cells. PMID: 29514220
2. Plexin C1 deficiency permits synaptotagmin 7-mediated macrophage migration and enhances mammalian lung fibrosis PMID: 27609773
3. loss of Plexin C1 in melanoma may promote early steps in melanoma progression through suppression of migration and proliferation PMID: 23160370
4. Study reports the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1; both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers. PMID: 20727575
5. GTP-bound form of Rab7 promotes melanogenesis through the regulation of gp100 maturation in melanoma cells. PMID: 17671519
6. Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression. PMID: 18987670
7. significant loss of Plexin C1 in metastatic melanoma compared with primary melanoma, suggesting the possibility that the Plexin C1 receptor is a tumor suppressor protein for melanoma PMID: 19318806
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Plexin family
組織特異性：

Detected in heart, brain, lung, spleen and placenta.
數據庫鏈接：

HGNC: 9106

OMIM: 604259

KEGG: hsa:10154

STRING: 9606.ENSP00000258526

UniGene: Hs.584845

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.