Human Peptidase inhibitor 16(PI16) ELISA kit

說明書下載

中文名稱：

人肽酶抑制劑16酶聯免疫試劑盒
貨號：

CSB-EL017951HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人肽酶抑制劑16酶聯免疫試劑盒（CSB-EL017951HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PI16含量。人肽酶抑制劑16是一種新型肽-肽酶抑制劑結合物，通過共價連接肽和肽酶抑制劑，延長肽的血清半衰期，提高藥物療效。其作用機制主要是通過抑制特定肽酶活性，延長肽類藥物在體內的作用時間，從而提高治療效果。試劑盒檢測范圍為0.312 ng/mL-10 ng/mL，該產品廣泛應用于基礎科研領域，如炎癥性疾病機制研究、感染模型中的宿主防御反應分析、皮膚或肺組織修復過程中的蛋白動態監測等，為探索Elafin在先天免疫調控及病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cripi ELISA Kit; CRISP 9 ELISA Kit; CRISP-9 ELISA Kit; CRISP9 ELISA Kit; Cysteine rich protease inhibitor ELISA Kit; Cysteine rich secretory protein 9 ELISA Kit; Cysteine-rich secretory protein 9 ELISA Kit; Microseminoprotein beta binding protein ELISA Kit; MSMBBP ELISA Kit; Peptidase inhibitor 16 ELISA Kit; PI-16 ELISA Kit; PI16 ELISA Kit; PI16_HUMAN ELISA Kit; Protease inhibitor 16 ELISA Kit; PSP94 binding protein ELISA Kit; PSP94-binding protein ELISA Kit; PSPBP ELISA Kit
縮寫：

PI16
Uniprot No.：

Q6UXB8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-10 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<20%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PI16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	83-98
1:2	Average %	95
	Range %	88-105
1:4	Average %	90
	Range %	84-105
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of human PI16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-105
EDTA plasma (n=4)	95	86-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10	2.254	2.266	2.260
5	1.342	1.354	1.348
2.5	0.662	0.669	0.666
0.625	0.197	0.211	0.204
0.312	0.099	0.102	0.101

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

May inhibit cardiomyocyte growth.
基因功能參考文獻：
1. at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
2. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation PMID: 22533972
3. A new blood protein PSP94-binding protein (PSPBP) is identified that binds prostate secretory protein of 94 amino acids (PSP94). PMID: 15344909
4. a novel and independent prognostic markers following radical prostatectomy for prostate cancer. PMID: 17062675
亞細胞定位：

Secreted.
蛋白家族：

CRISP family
組織特異性：

Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.
數據庫鏈接：

HGNC: 21245

KEGG: hsa:221476

STRING: 9606.ENSP00000362778

UniGene: Hs.25391

Human Peptidase inhibitor 16(PI16) ELISA kit

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