Human Odontogenic ameloblast-associated protein(ODAM) ELISA kit
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中文名稱：人牙成釉細(xì)胞相關(guān)蛋白(ODAM)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL016268HU
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人牙成釉細(xì)胞相關(guān)蛋白(ODAM)酶聯(lián)免疫試劑盒（CSB-EL016268HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ODAM含量。人牙成釉細(xì)胞相關(guān)蛋白(ODAM)是一種在牙齒發(fā)育中起關(guān)鍵作用的蛋白，其研究機(jī)制涉及調(diào)控成釉細(xì)胞分化過程，通過單細(xì)胞測(cè)序技術(shù)揭示了其時(shí)空表達(dá)模式，對(duì)牙齒發(fā)育和釉質(zhì)生成具有重要意義。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于口腔生物學(xué)、發(fā)育生物學(xué)等領(lǐng)域的基礎(chǔ)研究，例如探究牙齒發(fā)育機(jī)制、口腔疾病動(dòng)物模型中ODAM的動(dòng)態(tài)表達(dá)規(guī)律，或分析礦化組織相關(guān)蛋白調(diào)控網(wǎng)絡(luò)等科研需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：ODAM ELISA Kit; APIN ELISA Kit; Odontogenic ameloblast-associated protein ELISA Kit; Apin ELISA Kit
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縮寫：ODAM
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.04 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長：450 nm
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研究領(lǐng)域：Metabolism
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ODAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 104 Range % 99-109 1:10 Average % 98 Range % 95-102 1:20 Average % 84 Range % 80-88 1:40 Average % 90 Range % 85-94 -
回收率：
The recovery of human ODAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 93 89-97 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 2.682 2.790 2.736 2.564 5 2.079 2.005 2.042 1.870 2.5 1.310 1.254 1.282 1.110 1.25 0.773 0.729 0.751 0.579 0.625 0.458 0.423 0.441 0.269 0.312 0.374 0.367 0.371 0.199 0.156 0.257 0.269 0.263 0.091 0 0.171 0.173 0.172 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Use of odam as paradental disease biomarker P Joo-Cheol.et al,/,2018
- Odontogenic ameloblast-associated protein (ODAM) in gingival crevicular fluid for site-specific diagnostic value of periodontitis: a pilot study Hye-Kyung Lee.et al,BMC Oral Health,2018
- Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling. Lee HK .et al,J Biol Chem.,2015
- Parthenolide reduces empty lacunae and osteoclastic bone surface resorption induced by polyethylene particles in a murine calvarial model of peri??mplant osteolysis Zawawi MS et al,J Biomed Mater Res A,2015
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靶點(diǎn)詳情
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功能：Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.
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基因功能參考文獻(xiàn)：
- Risk of recurrence correlated with cytoplasmic expression of ODAM and pAKT, whereas nuclear expression did not predict recurrence of gastrointestinal stromal tumors. PMID: 27229116
- The inhibitory effects of ODAM on colorectal cancer cell growth were associated with PTEN elevation and PI3K/AKT signaling inactivation. PMID: 27694004
- ODAM induced RhoA activity. ODAM-mediated RhoA signalling resulted in actin filament rearrangement, increased cell adhesion and inhibited the migration/invasion of MCF7 cells. These results suggest that ODAM expression maintains their adhesion, resulting in the prevention of their metastasis via the regulation of RhoA signalling in breast cancer cells. PMID: 26358398
- We propose that ODAM has diverse functions that vary with protein location in various cell lines. Nuclear ODAM appears to be associated with MMP-20 regulation and tumorigenesis. Additional functions may take place in cytoplasm and extracellular matrix. PMID: 22387195
- Our studies demonstrate that ectopic ODAM expression in melanoma cell lines suppresses growth and migratory activity in these cells, while eliciting elevated PTEN expression and suppression of AKT activity. PMID: 23648148
- The expression of APIN at an early time-point following disruption of periodontal integrity suggests that this protein may be part of the cascade of events leading to the activation of ERM during periodontal healing and regeneration PMID: 20572917
- Calcifying epithelial odontogenic tumor-associated amyloid consists of a unique and previously undescribed protein that we provisionally designate APin. PMID: 14647039
- The protein sequences deduced from the cloned cDNA for rat, mouse, pig, and human were aligned together with those obtained from four other mammal genomes. Apin is highly conserved in mammals. PMID: 17647262
- Data suggest that ODAM is a developmental antigen that has an essential role in tooth maturation and in the pathogenesis of certain odontogenic and other epithelial neoplasms PMID: 18472969
- Confirmation of the amyloidogenic potential of ODAM has resulted from analyses of four other cases where we found, in addition, a 74-residue segment specified by exon 4. PMID: 18484335
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亞細(xì)胞定位：Secreted. Cytoplasm. Nucleus.
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蛋白家族：ODAM family
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組織特異性：Expressed in the junctional epithelium of healthy teeth. In periodontitis, absent in the pocket epithelium of the diseased periodontium but is detected in the gingival crevicular fluid.
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