Human Obestatin ELISA Kit

文獻引用 (4) 說明書下載

中文名稱：

人肥胖抑制素酶聯免疫試劑盒
貨號：

CSB-E12818h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人肥胖抑制素酶聯免疫試劑盒（CSB-E12818h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、尿液、組織勻漿樣本中的Obestatin含量。Obestatin是一種由胃部合成的23個氨基酸組成的酰胺化腦腸肽，與孤兒G蛋白偶聯受體GPR39結合，具有抑制食欲、減緩體重增加、抑制胃排空和小腸收縮的生物學功能。Obestatin與Ghrelin同源于Ghrelin基因，但作用相反，Obestatin可能通過GPR39受體調節胃腸動力。試劑盒檢測范圍為4.69 pg/mL-300 pg/mL，本試劑盒適用于體外科研場景，例如探究肥胖相關代謝疾病機制、胃腸功能調控或內分泌信號通路研究，研究者可通過標準曲線精確測定樣本中Obestatin的動態濃度變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Obestatin
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine, tissue homogenates
檢測范圍：

4.69 pg/mL-300 pg/mL
靈敏度：

1.17 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich
精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.
線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Obestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

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回收率：

The recovery of human Obestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Plasma levels of obestatin and ghrelin and obestatin/ghrelin ratio in obese patients M Sfar,ResearchGate,2023
Serum ghrelin and obestatin levels in patients with acne vulgaris: are they important for the severity? Zekiye Kanat, et al,Postepy Dermatologii I Alergologii,2019
Acute effect of smoking and smoking abstinence on energy intake and appetite-related hormones blood concentrations Yannakoulia M.et al,Physiol Behav,2017
Is association between thyroid hormones and gut peptides, ghrelin and obestatin, able to suggest new regulatory relation between the HPT axis and gut? Emami A et al,Regul Pept,2014

Human Obestatin ELISA Kit

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