Human Meteorin-like protein(METRNL) ELISA kit

Human Meteorin-like protein(METRNL) ELISA kit

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中文名稱：

人鎳紋樣蛋白(METRNL)酶聯免疫試劑盒
貨號：

CSB-EL013718HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人鎳紋樣蛋白(METRNL)酶聯免疫試劑盒（CSB-EL013718HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的METRNL含量。METRNL是一種新興靶點。其它與機體代謝、免疫調節等生理過程相關。研究機制上，它可能通過與特定受體結合，參與信號通路傳導，影響細胞的功能和多種生理活動，在糖尿病、炎癥等疾病研究中受關注。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，探究METRNL在代謝調節中的分子機制、評估肥胖相關模型動物或細胞中蛋白表達變化，以及炎癥信號通路與代謝疾病關聯性研究，為深入解析METRNL的生物學功能及其在代謝異常中的潛在作用提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Meteorin like glial cell differentiation regulator ELISA Kit; Meteorin; glial cell differentiation regulator like ELISA Kit; Meteorin-like protein ELISA Kit; METRL_HUMAN ELISA Kit; Metrnl ELISA Kit
縮寫：

METRNL
Uniprot No.：

Q641Q3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human METRNL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	90
	Range %	86-96
1:4	Average %	95
	Range %	90-99
1:8	Average %	93
	Range %	86-100

回收率：

The recovery of human METRNL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	92	88-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.695	2.587	2.641	2.471
5	2.079	2.136	2.108	1.938
2.5	1.682	1.604	1.643	1.473
1.25	1.266	1.169	1.218	1.048
0.625	0.719	0.694	0.707	0.537
0.312	0.399	0.427	0.413	0.243
0.156	0.218	0.209	0.214	0.044
0	0.173	0.167	0.170

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Interleukin-41 as a biomarker of the anti-inflammatory response associated with hyperuricemia S Zhang,Immunology letters,2023
Plasma interleukin-41 serves as a potential diagnostic biomarker for Kawasaki disease X Cai,Microvascular research,2023
Serum Metrnl Level is Correlated with Insulin Resistance, But Not with β-Cell Function in Type 2 Diabetics Wang C, et al,Medical Science Monitor,2019
Serum Levels of Meteorin-Like (Metrnl) Are Increased in Patients with Newly Diagnosed Type 2 Diabetes Mellitus and Are Associated with Insulin Resistance Wang K, et al,medical science monitor,2019

功能：

Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures.
基因功能參考文獻：
1. downregulation of METRNL during adipogenesis and functional induction of increased proliferation in stromal vascular fraction cells with concomitant inhibition of adipocyte differentiation may result in hypertrophic AT accumulation PMID: 27748745
2. Overexpression of METRNL inhibited mineralized nodule formation. PMID: 27716826
3. Data suggest that there is no correlation between serum Metrnl levels and BMI (body mass index) in humans. PMID: 26307585
4. Results show that Subfatin is a novel adipokine regulated by adipogenesis and obesity, with tissue distribution different from its homologue Meteorin PMID: 24393292
亞細胞定位：

Secreted.
蛋白家族：

Meteorin family
組織特異性：

Highly expressed in the skeletal muscle, in subcutaneous adipose tissue, epididymal white adipose tissue depots and heart. Also expressed in brown adipose tissues and kidney.
數據庫鏈接：

HGNC: 27584

OMIM: 616241

KEGG: hsa:284207

STRING: 9606.ENSP00000315731

UniGene: Hs.378821

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.