Human Layilin(LAYN) ELISA kit

Human Layilin(LAYN) ELISA kit

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中文名稱：

人排卵蛋白(LAYN)酶聯免疫試劑盒
貨號：

CSB-EL012772HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人排卵蛋白(LAYN)酶聯免疫試劑盒（CSB-EL012772HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LAYN含量。人排卵蛋白(LAYN)是一種調節T細胞功能的關鍵基因，其高表達與多種癌癥的預后不良相關，尤其是與胃癌和結腸癌的免疫浸潤水平密切相關。LAYN可能通過調節腫瘤相關巨噬細胞、DC、T細胞耗竭和Treg等免疫細胞在腫瘤微環境中的功能，影響腫瘤的進展和預后。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于體外研究LAYN在生殖生理調控、排卵障礙模型構建、卵巢功能評估以及免疫細胞相互作用等領域的表達特征，也可用于篩選調控LAYN表達的生物活性物質或藥物分子，為生殖醫學和免疫學基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Layilin ELISA Kit; LAYN ELISA Kit; LAYN_HUMAN ELISA Kit; UNQ208/PRO234 ELISA Kit
縮寫：

LAYN
Uniprot No.：

Q6UX15
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LAYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	92
	Range %	89-96
1:4	Average %	104
	Range %	101-107
1:8	Average %	89
	Range %	85-93

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.321

2.341

2.331

2.159

500

1.929

1.902

1.916

1.744

250

1.395

1.376

1.386

1.214

125

0.875

0.841

0.858

0.686

62.5

0.574

0.592

0.583

0.411

31.25

0.396

0.387

0.392

0.220

15.6

0.287

0.291

0.289

0.117

0.171

0.173

0.172

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Receptor for hyaluronate.
基因功能參考文獻：
1. Renal biopsy samples from patients with glomerulonephritis showed high expression of LAYN in tubular epithelial cells. PMID: 26410531
2. results indicate that LAYN would be involved in the enhancement of inflammation and degradation of cartilage in joint diseases such as RA and OA PMID: 25150153
3. Hyaluronan and layilin mediate loss of airway epithelial barrier function induced by cigarette smoke by decreasing E-cadherin. PMID: 23048036
亞細胞定位：

Membrane; Single-pass type I membrane protein.
數據庫鏈接：

HGNC: 29471

KEGG: hsa:143903

STRING: 9606.ENSP00000364764

UniGene: Hs.503831

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.